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LDH-Blue™ Cytotoxicity Assay

Lactate Dehydrogenase (LDH) Activity Assay Kit

LDH-Blue™ kit

Linear relationship between the number of lysed cells and the activity of released LDH

Pyroptotic cell death detection using LDH-Blue™

RSL3 induces LDH release in a dose-dependent manner

LDH-Blue™ allows kinetic or endpoint readings

#TLR7/8 Ligands

R848 (Resiquimod) - TLR7/8 Agonist

Imidazoquinoline compound - CAS #144875-48-9

NF-κB response of HEK-Blue™-derived cells to R848

NF-κB and IRF responses of THP1-Dual™-derived cells to R848

R848 chemical structure

HKST - TLR2/TLR4 Agonist

Heat Killed Salmonella typhimurium

HKST dose-dependent activation of TLR2 and TLR4

#TLR7/8 Ligands

TL8-506 - TLR8 Agonist

Benzo-azepine analog - CAS #1268163-15-0

NF-κB response of HEK-Blue™-derived cells to TL8-506

NF-κB and IRF responses of THP1-Dual™-derived cells to TL8-506

#TLR7/8 Ligands

ssPolyU Naked - TLR8 Agonist

RNA homopolymer

NF-κB response of HEK-Blue™-derived cells to ssPolyU Naked

#TLR7/8 Ligands

ORN06/LyoVec™ - TLR7/8 Agonist

UUGU ssRNA polymer / LyoVec™ complexed

NF-κB response of HEK-Blue™-derived cells to ORN06/LyoVec™

Pam3CSK4 Biotin - TLR2/TLR1 Agonist

Biotinylated Synthetic triacylated lipopeptide

Pam3CSK4 Biotin dose-dependent activation of TLR2

Pam3CSK4 - TLR2/TLR1 Agonist

Synthetic triacylated lipopeptide

Evaluation of NF-κB activation with Pam3CSK4

Specificity of Pam3CSK4 activation

Pam2CSK4 - TLR2/TLR6 Agonist

Synthetic diacylated lipopeptide

Discrimination of TLR1-TLR2 and TLR2-TLR6 activation with synthetic ligands and heat-killed bacteria

FSL-1 - TLR2/TLR6 Agonist

Synthetic diacylated lipoprotein

Dose-dependent activation of TLR2 by FSL-1

PGN-SA peptidoglycan preparation - TLR2 Agonist

PGN from Staphylococcus aureus

PGN-SA dose-dependent activation of TLR2

PGN-BS peptidoglycan preparation - TLR2 Agonist

PGN from Bacillus subtilis

Response of HEK-Blue™ hTLR2 to TLR2 agonists

LTA-SA - TLR2 Agonist

LTA from S. aureus

Response of HEK-Blue™ hTLR2 to TLR2 agonists

HKSP - TLR2 Agonist

Heat Killed - Streptococcus pneumoniae

HKSP dose-dependent activation of TLR2

HKSA - TLR2 Agonist

Heat Killed - Staphylococcus aureus

HKSA dose-dependent activation of TLR2

#TLR7/8 Ligands

Loxoribine - TLR7 Agonist

Guanosine analog - CAS #121288-39-9

NF-κB response of HEK-Blue™-derived cells to loxoribine

#TLR7/8 Ligands

Imiquimod (R837) - TLR7 Agonist

Imidazoquinoline compound - CAS #99011-78-6

NF-κB response of HEK-Blue™-derived cells to imiquimod

NF-κB and IRF responses of HEK-Dual™-derived cells to imiquimod

#TLR7/8 Ligands

Gardiquimod™ - TLR7 Agonist

Imidazoquinoline compound - CAS #1020412-43-4

NF-κB response of HEK-Blue™-derived cells to Gardiquimod™

NF-κB and IRF responses of THP1-Dual™-derived cells to Gardiquimod™

#TLR7/8 Ligands

CL264 - TLR7 Agonist

Adenine analog

NF-κB response of HEK-Blue™-derived cells to CL264

NF-κB and IRF responses of HEK-Dual™-derived cells to CL264

#TLR7/8 Ligands

CL097 - TLR7/TLR8 Agonist

Imidazoquinoline compound - CAS #1026249-18-2

NF-κB response of HEK-Blue™-derived cells to CL097

NF-κB and IRF responses of THP1-Dual™-derived cells to CL097