LTA-SA

Lipoteichoic acid from S. aureus

Lipoteichoic acid (LTA) is a major immunostimulatory component of Gram-positive bacteria. Like LPS, LTA is an amphiphile formed by a hydrophilic polyphosphate polymer linked to a neutral glycolipid.

LTA stimulates immune cells through TLR2 to produce TNF-α and other inflammatory cytokines. Recognition of LTA involves also LPS-binding protein (LBP) and CD14.

InvivoGen provides LTA purified from S. aureus (LTA-SA) with the EndoFit™ grade (<0.001 EU/μg).

Specifications

Specificity: TLR2 agonist
Working concentration: 1 ng -1 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/μg)

Contents

• 5 mg lipoteichoic acid (LTA-SA) purified from S. aureus 
• 10 ml endotoxin-free water

LTA-SA is shipped at room temperature

Stored at -20 ̊C. Upon resuspension, store LTA-SA at 4 ̊C for short-term storage or at -20 ̊C for long storage.

Avoid repeated freeze-thaw cycles.

 The product is stable for 1 month at 4̊C and 6 months at -20 ̊C when properly stored.

Description

Lipoteichoic acid (LTA) is a major immunostimulatory component of Gram-positive bacteria. LTA shares many of the biochemical and physiological properties of the LPS of gram-negative bacteria. LTA is responsible for causing gram-positive sepsis. Like LPS, LTA is an amphiphile formed by a hydrophilic polyphosphate polymer linked to a neutral glycolipid. LTA stimulates immune cells through TLR2 to produce TNF-α and other inflammatory cytokines [1].

LTA-SA Purified contains 10 times less endotoxin according to the gel clot LAL Assay than the standard preparation. At concentrations ranging from 1 ng to 10 μg/ml, it highly activates TLR2 and no other TLRs including TLR4.

1. Schwandner R. et al., 1999. Peptidoglycan- and lipoteichoic acid-induced cell activation is mediated by toll-like receptor 2. J Biol Chem, 274(25):17406-9.