PGN-BS
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Cat.code:
tlrl-pgnb3
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ABOUT
Peptidoglycan from B. subtilis
PGN-BS is a peptidoglycan (PGN) preparation from the Gram-positive Bacillus subtilis.
PGN is a major component of the bacterial cell wall. It is mainly single-layered in Gram-negative bacteria (e.g. E. coli) and multi-layered in Gram-positive bacteria (e.g. B. subtilis). PGN is a large polymeric molecule made of glycan strands connected by short peptides. PGN structure varies among bacterial species at the level of sugar polymers, and of cross-linked amino-acids. In Gram-positive bacteria, the amino-acid side chain structure consists of L-alanine, D-glutamate, L-lysine, and D-alanine. In Gram-negative bacteria, the L-Lysine is replaced by meso-diaminopimelic acid (mDAP or meso-DAP) [1].
PGN, either intact or fragmented, is sensed by the innate immune system through different PRRs (pattern recognition receptors). PGN is known to be a potent activator of NF-κB and TNF-α through TLR2, although it has been reported that PGN agonist activity for TLR2 is likely relying on other commonly co-purified molecules, such as cell wall lipoproteins and lipoteichoic acids (LTAs). The role of TLR2 in the direct recognition of PGN remains controversial, and the discrepancies among studies may arise from the purification methods as well as from the variation in PGN structure in the different bacterial species [1].
KEY FEATURES OF PGN-EB:
- Preparation of peptidoglycan from Bacillus subtilis
- Functionally-tested using cellular reporter assays
- Potent TLR2 agonist
- Potent NOD2 agonist
InvivoGen also offers ultrapure PGNs as NOD1/NOD2 agonists, and which do not activate TLR2 nor TLR4.
Reference:
1. Wolf A.J. & Underhill D.M., 2018. Peptidoglycan recognition by the innate immune system. Nat. Rev. Immunol. 18(4):243-254.
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
- 30 ng/ml - 10 µg/ml for human TLR2 in cellular assays
- 3 - 10 μg/ml for human NOD2 in cellular assays
Cellular assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:PGN-BS
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Cat code:tlrl-pgnb3
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Quantity:5 mg
25 ml of endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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