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PGN-ECndi ultrapure

PGN-ECndi ultrapure, insoluble Unit size Cat. code Docs Qty Price
Insoluble peptidoglycan from E. coli K12
5 mg
tlrl-kipgn
+-
$215.00

Insoluble peptidoglycan from E. coli K12

Peptidoglycan (PDN) is a major surface component of Gram-positive bacteria. It is embedded in a relatively thick cell wall and is usually covalently attached to other polymers, such as lipoproteins and LTAs.

PGN is known to be a potent activator of NF-κB and TNF-α through TLR2 [1].

However, other pattern recognition proteins have been reported to mediate the immunostimulatory activity of PGN [2, 3, 4]. This discrepancy is correlated to the method of purification. According to Travassos et al., the presence of these polymers in most preparations of PGN is responsible for the TLR2-dependent activity of PGN [2].

PGNs purified by detergent lysis and hydrolysis under basic conditions to eliminate lipophilic constituents lose their ability to activate TLR2-transfected HEK293 cells but still activate NOD2-transfected cells.

 

References:

1. Takeuchi O. et al. 1999. Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall copmonents. Immunity, 11(4):443-51.
2. Travassos.LH. et al., 2004. Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. EMBO Rep. 5(10):1000-1006.
3.Girardin SE et al., 2003. Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2. J Biol Chem. 278(43):41702-8.
4. Dziarski R., 2004. Peptidoglycan recognition proteins (PGRPs). Mol Immunol. 40(12): 877-86.

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Specifications

Specificity: NOD1/NOD2 agonist

Working Concentration: 1 - 5 µg/ml

Resuspension: 200 μg/ml in water.

Note: PGN-ECndi ultrapure is insoluble. The suspension appears hazy.

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Contents

PGN-ECndi is provided as a lyophilized white solid.

  • 5 mg insoluble peptidoglycan from E. coli K12 (PGN-ECndi)
  • 25 ml sterile endotoxin-free water

room temperature PGN-ECndi is shipped at room temperature.

store Store at -20°C. Resuspended PGN-ECndi can be stored at 4°C for 1 month or at -20°C for at least 6 months.

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Citations

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