PGN-SAndi ultrapure peptidoglycan

Product Unit size Cat. code Docs. Qty. Price

PGN-SAndi ultrapure, insoluble

Insoluble peptidoglycan from S. aureus

Show product

5 mg

  • About
  • Specifications
  • Contents
  • Related products

Insoluble Peptidoglycan from Staphylococcus aureus

PGN-SAndi is an ultrapure preparation of peptidoglycan (PGN) from the Gram-positive Staphylococcus aureus.

PGN is a major component of the bacterial cell wall. It is mainly single-layered in Gram-negative bacteria (e.g. E. coli) and multi-layered in Gram-positive bacteria (e.g. B. subtilis). PGN is a large polymeric molecule made of glycan strands connected by short peptides. PGN structure varies among bacterial species at the level of sugar polymers, and of cross-linked amino-acids. In Gram-positive bacteria, the amino-acid side chain structure consists of L-alanine, D-glutamate, L-lysine, and D-alanine. In Gram-negative bacteria, the L-Lysine is replaced by meso-diaminopimelic acid (mDAP or meso-DAP) [1].

PGN, either intact or fragmented, is sensed by the innate immune system through different PRRs (pattern recognition receptors). PGN is known to be a potent activator of NF-κB and TNF-α through TLR2, although it has been reported that PGN agonist activity for TLR2 is likely relying on other commonly co-purified molecules, such as cell wall lipoproteins and lipoteichoic acids (LTAs). The role of TLR2 in the direct recognition of PGN remains controversial, and the discrepancies among studies may arise from the purification methods as well as from the variation in PGN structure in the different bacterial species [1-2].

PGN-SAndi ultrapure from S. aureus is an insoluble preparation of PGN purified by detergent lysis and hydrolysis under basic conditions to eliminate lipophilic constituents. This PGN preparation does not activate TLR2-transfected HEK293 cells, but it still activates NOD2-transfected cells. 



  • Ultrapure preparation of peptidoglycan from Staphylococcus aureus
  • Insoluble
  • Functionally-tested using cellular reporter assays
  • NOD2 agonist



1. Wolf A.J. & Underhill D.M., 2018. Peptidoglycan recognition by the innate immune system. Nat. Rev. Immunol. 18(4):243-254.
2. Travassos.LH. et al., 2004. Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. EMBO Rep. 5(10):1000-1006.

Back to the top


Specificity: NOD2 agonist

Working Concentration: 1 - 10 µg/ml

Resuspension: 200 μg/ml in waterNote: PGN-SAndi ultrapure is insoluble. The suspension appears hazy.

Quality control:

Back to the top


  • 5 mg of insoluble peptidoglycan from Staphylococcus aureus (PGN-SAndi) ultrapure
  • 25 ml of sterile endotoxin-free water

room temperature PGN-SAndi ultrapure is shipped at room temperature.

store Upon receipt, store at -20°C. 

Alert Avoid repeated freeze-thaw cycles.

Back to the top
Customer Service
& Technical Support
Shopping cart is empty