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M-TriDAP

M-TriDAP Unit size Cat. code Docs Qty Price
NOD1/NOD2 ligand
1 mg
tlrl-mtd
+-
$124.00

MurNAc-L-Ala-γ-D-Glu-mDAP (M-TriDAP), also called DAP-containing muramyl tripeptide is a peptidoglycan (PGN) degradation product found mostly in Gram-negative bacteria.

M-TriDAP is recognized by the intracellular sensor NOD1 (CARD4) and to a lesser extend NOD2 (CARD15). Recognition of M-TriDAP by NOD1/NOD2 induces a signaling cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 [1]. M-TriDAP induces the activation of NF-κB at similar levels to Tri-DAP [2].

M-TriDAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1 and HEK-Blue™ NOD2 Cells.

Note: M-TriDAP is a mixture of MurNAc-L-Ala-γ-D-Glu-D- mDAP and MurNAc-L-Ala-γ-D-Glu-L-mDAP

 

1. Park JH. et al., 2007. RICK/RIP2 mediates innate immune responses induced through Nod1 and Nod2 but not TLRs. J Immunol. 178(4):2380-6.
2. Girardin SE. et al., 2003. Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2. J Biol Chem. 278(43):41702-8.

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Specifications

Specificity: NOD1/NOD2 agonist

Working Concentration: 100 ng - 10 µg/ml

Molecular weight: 665.64

Synonym: N-acetyl-muramyl-L-Ala-γ-D-Glu-meso- diaminopimelic acid

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Contents

M-TriDAP is provided as a lyophilized powder.

  • 1 mg M-TriDAP
  • 1.5 ml endotoxin-free water

room temperature M-TriDAP is shipped at room temperature.

store Store at -20°C.

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Citations

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