M-TriDAP - NOD1/NOD2 Agonist

NOD1/NOD2 Agonist - MurNAc-L-Ala-gamma-D-Glu-mDAP - PGN-like molecule

MurNAc-L-Ala-γ-D-Glu-mDAP (M-TriDAP), also called DAP-containing muramyl tripeptide is a peptidoglycan (PGN) degradation product found mostly in Gram-negative bacteria.

M-TriDAP is recognized by the intracellular sensor NOD1 (CARD4) and to a lesser extend NOD2 (CARD15).

Recognition of M-TriDAP by NOD1/NOD2 induces a signaling cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNF-α and IL-6 [1]. M-TriDAP induces the activation of NF-κB at similar levels to Tri-DAP [2].

M-TriDAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1 and HEK-Blue™ NOD2 Cells.

References:

1. Park JH. et al., 2007. RICK/RIP2 mediates innate immune responses induced through Nod1 and Nod2 but not TLRs. J Immunol. 178(4):2380-6.
2. Girardin SE. et al., 2003. Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2. J Biol Chem. 278(43):41702-8.