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PGN-ECndss ultrapure

PGN-ECndss ultrapure, soluble Unit size Cat. code Docs Qty Price
Soluble sonicated peptidoglycan from E. coli
1 ml (1 mg/ml)
tlrl-ksspgn
+-
$215.00

Soluble sonicated peptidoglycan from E. coli K12

Gram positive peptidoglycan (PGN) is usually bound to other polymers such as teichoic acids and carbohydrates, while Gram negative PGN is commonly attached covalently to a single type of lipoprotein.

According to Travassos et al., the presence of these polymers in most preparations of PGNs is responsible for the TLR2-dependent activity of PGN [1].

PGNs purified by detergent lysis and hydrolysis under basic conditions to eliminate lipophilic constituents lose their ability to activate TLR2-transfected HEK293 cells but still activate NOD2-transfected cells. Insoluble PGN from E. coli also activates NOD1-transfected cells.

Soluble PGN from E. coli K12 is obtained after sonication of insoluble PGNs. At the working concentrations, it activates  HEK-Blue™ NOD1 and HEK-Blue™ NOD2 cells but not HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

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Specifications

Specificity: NOD1/NOD2 agonist

Working Concentration: 1 - 5 µg/ml

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Contents

PGN-ECndss is provided as a solution at 1 mg/ml

  • 1 ml of soluble sonicated peptidoglycan from Escherichia coli K12 (PGN-ECndss)
  • 1.5 ml of sterile endotoxin-free water

room temperature PGN-ECndss is shipped at room temperature.

store PGN-ECndss can be stored at room temperature for 2 weeks and at 4°C for up to 6 months.

Note: When stored at 4°C, PGN-ECndss may precipitate. Place the product in a water bath at 37°C for a 5 minutes and homogenize for complete solubilization.

Alert Do not freeze. PGN-ECndss will not return to solubility.

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Citations

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