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HEK TLR Reporter Cells

InvivoGen offers a collection of HEK293-derived TLR (Toll-like receptor) reporter cells designed for rapid, sensitive, and reliable screening of TLR agonists or antagonists. They allow the monitoring of TLR-induced NF-κB and/or IRF signaling.
 

HEK-Blue™ TLR cells (NF-κB)

  • Stable expression of a human or mouse TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
     

HEK-Blue-Lucia™ TLR cells* (NF-κB/IL8)

  • Stable expression of a human or mouse TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
  • Stable expression of the Lucia luciferase reporter gene under the control of the endogenous IL-8 promoter (responsive to NF-κB)
  • No activity of TLR3, TLR5, and TNFR (Tumor Necrosis Factor receptor) due to verified knockout of these three receptors

​*Note: these cells were formerly named HEK-Dual™ TLR (NF/IL8) cells.
 

HEK-Dual™ TLR cells (NF-κB/IRF)

  • Stable expression of a human TLR gene
  • Stable expression of an NF-κB/AP1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene
  • Stable expression of an IRF-inducible Lucia luciferase reporter gene

 

 

 

The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents, QUANTI-Blue™ Solution or HEK-Blue™ Detection, and the Lucia luciferase detection reagent, QUANTI-Luc™ 4 Lucia/Gaussia.

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