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pFUSE-CHIg-hG1

Product Unit size Cat. code Docs. Qty. Price

pFUSE-CHIg-hG1

Human IgG1

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20 µg

pfuse-hchg1
+-
$684

pFUSEss-CHIg-hG1

Human IgG1

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20 µg

pfusess-hchg1
+-
$684
  • About
  • Specifications
  • pFUSE Contents
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pFUSE-CHIg
pFUSE-CHIg general plasmid map

Human IgG1 heavy chain constant region

 

pFUSE-CHIg-hG1 is a cloning plasmid that expresses the constant region of the human IgG1 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

 

Key features of pFUSE-CHIg-hG1

  • Encodes the human IgG1 CH constant region
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin® in both bacterial and mammalian cells
  • Available with or without the IL-2 signal sequence
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Specifications

• pFUSE-CHIg-hG1 features the constant region of the human IgG1 heavy chain

 

pFUSEss-CHIg-hG1 features the constant region of the human IgG1 heavy chain and the IL2 signal sequence

Both plasmids are selectable with Zeocin®.

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pFUSE Contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin® (100 mg/ml)

room temperature Product is shipped at room temperature

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