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pFUSE-CHIg-hG2

pFUSE-CHIg-hG2 Unit size Cat. code Docs Qty Price
Human IgG2
20 µg
pfuse-hchg2
+-
$536.00
pFUSEss-CHIg-hG2 Unit size Cat. code Docs Qty Price
Human IgG2
20 µg
pfusess-hchg2
+-
$536.00

pFUSE-CHIg-hG2 is a cloning plasmid that expresses the constant region of the human IgG2 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

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Specifications

• pFUSE-CHIg-hG2 features the constant region of the human IgG2 heavy chain

pFUSEss-CHIg-hG2 features the constant region of the human IgG2 heavy chain and the IL2 signal sequence

Both plasmids are selectable with Zeocin™.

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Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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Citations

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