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pFUSE-CHIg-hM

pFUSE-CHIg-hM Unit size Cat. code Docs Qty Price
Human IgM allele 3
20 µg
pfuse-hchm3
+-
$612.00
pFUSEss-CHIg-hM Unit size Cat. code Docs Qty Price
Human IgM allele 3
20 µg
pfusess-hchm3
+-
$612.00

pFUSE-CHIg
pFUSE-CHIg general plasmid map

Human IgM heavy chain constant region

 

pFUSE-CHIg-hM is a cloning plasmid that expresses the constant region of the human IgM heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hM additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

 

Key features of pFUSE-CHIg-hM

  • Encodes the human IgM CH constant region
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin® in both bacterial and mammalian cells
  • Available with or without the IL-2 signal sequence
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Specifications

• pFUSE-CHIg-hM features the constant region of the human IgM heavy chain

 

pFUSEss-CHIg-hM features the constant region of the human IgM heavy chain and the IL2 signal sequence

Both plasmids are selectable with Zeocin®.

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pFUSE Contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin® (100 mg/ml)

room temperature Product is shipped at room temperature

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