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pFUSE2-CLIg-hK

pFUSE2-CLIg-hK Unit size Cat. code Docs Qty Price
Human kappa
20 µg
pfuse2-hclk
+-
$612.00
pFUSE2ss-CLIg-hK Unit size Cat. code Docs Qty Price
Human kappa
20 µg
pfuse2ss-hclk
+-
$612.00

Light chain expression plasmid - Human kappa

pFUSE2-CLIg-hK is a cloning plasmid that expresses the constant region of the human kappa light chain. It contains a multiple cloning site upstream of the constant region to enable cloining of the light chain variable region.

This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.

pFUSE2ss-CLIg-hK additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

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Specifications

  • pFUSE2-CLIg-hK features the constant region of the human Ig kappa light chain
  • pFUSE2ss-CLIg-hK features the constant region of the human Ig kappa light chain and the IL2 signal sequence

Both plasmids are selectable with blasticidin.

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pFUSE2 Contents

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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Citations

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