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Human IgG4

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20 µg



Human IgG4 with IL2ss

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20 µg

  • About
  • Specifications
  • pFUSE Contents
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pFUSE-CHIg general plasmid map

Human IgG4 heavy chain constant region


pFUSE-CHIg-hG4 is a cloning plasmid that expresses the constant region of the human IgG4 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG4 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.


Key features of pFUSE-CHIg-hG4

  • Encodes the human IgG4 CH constant region
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin® in both bacterial and mammalian cells
  • Available with or without the IL-2 signal sequence
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• pFUSE-CHIg-hG4 features the constant region of the human IgG4 heavy chain

pFUSEss-CHIg-hG4 features the constant region of the human IgG4 heavy chain and the IL2 signal sequence

Both plasmids are selectable with Zeocin®.

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pFUSE Contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin® (100 mg/ml)

room temperature Product is shipped at room temperature

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