pFUSE-CHIg-hG4

Human IgG4 heavy chain constant region

pFUSE-CHIg-hG4 is a cloning plasmid that expresses the constant region of the human IgG4 heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG4 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Key features of pFUSE-CHIg-hG4

• Encodes the human IgG4 CH constant region
• Long-lasting expression due to the composite hEF1-HTLV promoter
• Selectable with Zeocin® in both bacterial and mammalian cells
• Available with or without the IL-2 signal sequence