pFUSE-CHIg-hE
| pFUSE-CHIg-hE | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Human IgE allele 2 |
20 µg |
pfuse-hche2 |
| pFUSEss-CHIg-hE | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Human IgE allele 2 |
20 µg |
pfusess-hche2 |
pFUSE-CHIg-hE is a cloning plasmid that expresses the constant region of the human IgE heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-hE additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Specifications
• pFUSE-CHIg-hE features the constant region of the human IgE heavy chain
• pFUSEss-CHIg-hE features the constant region of the human IgE heavy chain and the IL2 signal sequence
Both plasmids are selectable with Zeocin™.
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• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)