pFUSE-CHIg-mE
| pFUSE-CHIg-mE | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Mouse IgE |
20 µg |
pfuse-mche |
| pFUSEss-CHIg-mE | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Mouse IgE |
20 µg |
pfusess-mche |
pFUSE-CHIg general plasmid map
Murine IgE heavy chain constant region
pFUSE-CHIg-mE is a cloning plasmid that expresses the constant region of the mouse IgE heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.
pFUSEss-CHIg-mE additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.
Key features of pFUSE-CHIg-mE
- Encodes the murine IgE CH constant region
- Long-lasting expression due to the composite hEF1-HTLV promoter
- Selectable with Zeocin™ in both bacterial and mammalian cells
- Available with or without the IL-2 signal sequence
Specifications
• pFUSE-CHIg-mE features the constant region of the mouse IgE heavy chain
• pFUSEss-CHIg-mE features the constant region of the mouse IgE heavy chain and the IL2 signal sequence
Both plasmids are selectable with Zeocin™.
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- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature