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pFUSE2-CLIg-mL2

pFUSE2-CLIg-mL2 Unit size Cat. code Docs Qty Price
Mouse lambda 2
20 µg
pfuse2-mcll2
+-
$536.00
pFUSE2ss-CLIg-mL2 Unit size Cat. code Docs Qty Price
Mouse lambda 2
20 µg
pfuse2ss-mcll2
+-
$536.00

pFUSE2-CLIg-mL2 is a cloning plasmid that expresses the constant region of the mouse lambda 2 light chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the light chain variable region.

This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.

pFUSE2ss-CLIg-mL2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

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Specifications

  • pFUSE2-CLIg-mL2 features the constant region of the mouse Ig lambda 2 light chain
  • pFUSE2ss-CLIg-mL2 features the constant region of the rabbit Ig mouse lambda 2 light chain and the IL2 signal sequence

Both plasmids are selectable with blasticidin.

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Contents

  • 20 μg of lyophilized DNA
  • 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stable Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

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