pFUSE-CHIg-mG2b

Heavy chain expression plasmid - Murine IgG2b

ABOUT

Murine IgG2b heavy chain constant region

 

pFUSE-CHIg-mG2b is a cloning plasmid that expresses the constant region of the mouse IgG2b heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-mG2b additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

 

Key features of pFUSE-CHIg-mG2b

  • Encodes the murine IgG2b CH constant region
  • Long-lasting expression due to the composite hEF1-HTLV promoter
  • Selectable with Zeocin™ in both bacterial and mammalian cells
  • Available with or without the IL-2 signal sequence

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Gene
Mouse IgG2b heavy chain
Species
Mouse
Plasmid backbone
pFUSE-CHIg
Antibiotic resistance
Zeocin®
Gene promoter
Human EF1α/HTLV hybrid promoter
Reconstitution buffer
Sterile water (not provided)
Tested applications

in vitro transfection

Quality control

Plasmid construct is confirmed by restriction analysis and full-length open reading frame (ORF) sequencing.

CONTENTS

Contents

  • Product: 
    pFUSE-CHIg-mG2b
  • Cat code: 
    pfuse-mchg2b
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: Stable up to 1 year when stored at -20°C

DOCUMENTS

Documents

pFUSE-CHIg-mG2b

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

Need a CoA ?

CUSTOMER SERVICE & TECHNICAL SUPPORT

Question about this product ?