LumiKine™ Xpress hIFN-β 2.0
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Cat.code:
luex-hifnbv3
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The concentrations of the hIFN-β standard and Lucia-conjugated detection antibody have been modified. Please follow the updated reconstitution instructions in the TDS.
ABOUT
Rapid bioluminescent human IFN-β ELISA kit
LumiKine™ Xpress hIFN-β 2.0 is InvivoGen’s second-generation bioluminescent human interferon-beta (hIFN-β) ELISA, using a Lucia luciferase-conjugated detection antibody. LumiKine™ Xpress hIFN-β 2.0 is an optimized ‘sandwich’ ELISA kit designed to rapidly and reliably measure the concentration of hIFN-β in various biological samples (cell culture supernatant, serum, and plasma).
After a single 2-hour incubation, the detection of hIFN-β is instantaneously determined using QUANTI-Luc™4 Reagent, a Lucia luciferase detection reagent. The bioluminescent signal is readily assessed by luminometry.
Features of LumiKine™ Xpress hIFN-β 2.0
- Single incubation: 2 hours
- Limit of Detection: 10 pg/ml
- Specificity: Human IFN-β
- Optimized antibody pairing: The capture and detection antibodies bind to different epitopes of the cytokine
- No cross-reactivity with hIFN-α2, murine (m)IFN-α2 or mIFN-β
LumiKine™ Xpress hIFN-β 2.0 is provided with a standard recombinant hIFN-β produced in mammalian cells, to ensure the most accurate and sensitive calibration for the measurement of unknown levels of hIFN-β.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Quantification of cytokine levels in multiple sample types
IFN-β
Human
50 µl
10 pg/ml
Quantification of human IFN-β in serum sample
Each lot is functionally tested and validated.
No cross-reactivity with human IFN-α, mouse (m)IFN-α2, or mIFN‑β
CONTENTS
Contents
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Product:LumiKine™ Xpress hIFN-β 2.0
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Cat code:luex-hifnbv3
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Quantity:5 plates
- 150 µg lyophilized hIFN-β capture antibody
- 5 µg lyophilized Lucia-conjugated detection antibody
- 100 ng lyophilized hIFN-β standard (CHO-expressed recombinant cytokine)
- 2 tubes of QUANTI-Luc™ 4 Reagent (sufficient to prepare 50 ml)
- 5 white flat-bottom MaxiSorp® 96-well plates with 5 plate sealers
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
- Protect from light
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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