LumiKine™ Xpress mIFN-β

Mouse IFN-beta bioluminescent ELISA kit

ABOUT

Rapid bioluminescent mouse IFN-β ELISA kit

LumiKine™ Xpress mIFN-β 2.0 is InvivoGen’s second-generation bioluminescent murine interferon-beta (mIFN-β) ELISA, using a Lucia luciferase-conjugated detection antibody. LumiKine™ Xpress mIFN-β 2.0 is an optimized ‘sandwich’ ELISA kit designed to rapidly and reliably measure the concentration of mIFN-β in various biological samples (cell culture supernatant, serum, and plasma).

After a single 2-hour incubation, the detection of mIFN-β is instantaneously determined using QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent. The bioluminescent signal is readily assessed by luminometry.

Features of LumiKine™ Xpress mIFN-β 2.0:

  • Single incubation: 2 hours
  • Limit of Detection: 5 pg/ml
  • Specificity: Mouse IFN-β
  • Optimized antibody pairing: The capture and detection antibodies bind to different epitopes of the cytokine
  • No cross-reactivity with human (h)IFN-α2, hIFN-β or mIFN-α2

LumiKine™ Xpress mIFN-β 2.0 is provided with a standard recombinant mIFN-β produced in mammalian cells, to ensure the most accurate and sensitive calibration for the measurement of unknown levels of mIFN-β.

All InvivoGen products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Quantification of cytokine levels in multiple sample types

Target

IFN‑β

Target species

Mouse

Sample types
Cell culture supernatant, serum, plasma
Sample volume

50 µl

Limit of detection

5 pg/ml

Assay time
2 hours (after plate preparation)
Detection method
Bioluminescence
Tested applications

Quantification of mouse IFN-β in serum sample

Quality control

Each lot is functionally tested and validated.

Additional information

No cross-reactivity with mouse IFN-α2, human (h)IFN-α2 or hIFN‑β

CONTENTS

Contents

  • Product: 
    LumiKine™ Xpress mIFN-β 2.0
  • Cat code: 
    luex-mifnbv3
  • Quantity: 
    5 plates
Includes:
  • 150 µg lyophilized mIFN-β capture antibody
  • 5 µg lyophilized Lucia-conjugated detection antibody
  • 100 ng lyophilized mIFN-β standard
  • 2 tubes of QUANTI-Luc™ 4 Reagent (sufficient to prepare 50 ml)
  • 5 white flat-bottom MaxiSorp® 96-well plates, 5 plate sealers

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: See TDS

    Caution:

    • Avoid repeated freeze-thaw cycles
    • Protect from light

DOCUMENTS

Documents

LumiKine™ Xpress mIFN-β 2.0

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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