LumiKine™ Xpress mIFN-α

Mouse IFN-alpha bioluminescent ELISA kit

ABOUT

Rapid bioluminescent mouse IFN-α ELISA kit

LumiKine™ Xpress mIFN-α 2.0 is InvivoGen’s second-generation bioluminescent murine interferon-alpha (mIFN-α) ELISA, using a Lucia luciferase-conjugated detection antibody. LumiKine™ Xpress mIFN-α 2.0 is an optimized ‘sandwich’ ELISA kit designed to rapidly and reliably measure the concentration of mIFN-α in various biological samples (cell culture supernatant, serum, and plasma).

After a single 2-hour incubation, the detection of mIFN-α is instantly determined using QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent. The bioluminescent signal is readily assessed by luminometry.

Features of LumiKine™ Xpress mIFN-α 2.0:

  • Single incubation: 2 hours
  • Limit of Detection: 7 pg/ml
  • Specificity: Murine IFN-α2
  • Optimized antibody pairing: The capture and detection antibodies bind to different epitopes of the cytokine
  • No cross-reactivity with human (h)IFN-α2, hIFN-β, or mIFN-β

LumiKine™ Xpress mIFN-α 2.0 is provided with a standard recombinant mIFN-α2 produced in mammalian cells, to ensure the most accurate and sensitive calibration for the measurement of unknown levels of mIFN-α.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Quantification of cytokine levels in multiple sample types.

Target

IFN-α

Target species

Mouse

Sample types
Cell culture supernatant, serum, plasma
Sample volume

50 µl

Limit of detection

7 pg/ml

Assay time
2 hours (after plate preparation)
Detection method
Bioluminescence
Tested applications

Quantification of mIFN-α in serum sample

Quality control

Each lot is functionally tested and validated.

Additional information

No cross-reactivity with mouse IFN‑β, human (h)IFN-α2 or hIFN‑β

CONTENTS

Contents

  • Product: 
    LumiKine™ Xpress mIFN-α 2.0
  • Cat code: 
    luex-mifnav2
  • Quantity: 
    5 plates
Includes:
  • 150 µg lyophilized mIFN-α capture antibody
  • 5 µg lyophilized Lucia-conjugated detection antibody
  • 100 ng lyophilized mIFN-α2 standard (HEK293-expressed recombinant cytokine)
  • 2 tubes of QUANTI-Luc™ 4 Reagent (sufficient to prepare 50 ml)
  • 5 white flat-bottom MaxiSorp® 96-well plates with 5 plate sealers

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: See TDS

    Caution:

    • Avoid repeated freeze-thaw cycles
    • Protect from light

DOCUMENTS

Documents

LumiKine™ Xpress mIFN-α 2.0

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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