LumiKine™ Xpress hIFN-α

Human IFN-alpha bioluminescent ELISA kit

ABOUT

Rapid bioluminescent human IFN-α2 ELISA kit

LumiKine™ Xpress hIFN-α 2.0 is InvivoGen’s second-generation bioluminescent human interferon-alpha (hIFN-α) ELISA, using a Lucia luciferase-conjugated detection antibody. LumiKine™ Xpress hIFN-α 2.0 is an optimized ‘sandwich’ ELISA kit designed to rapidly and reliably measure the concentration of hIFN-α2 in various biological samples (cell culture supernatant, serum, and plasma).

After a single 2-hour incubation, the detection of hIFN-α2 is instantly determined using QUANTI-Luc™ 4 Reagent, a Lucia luciferase detection reagent. The bioluminescent signal is readily assessed by luminometry.


Features of LumiKine™ Xpress hIFN-α 2.0:

  • Single incubation: 2 hours
  • Limit of Detection: 5 pg/ml
  • Specificity: hIFN-α2 (multiple subtypes can also be detected)
  • Optimized antibody pairing: The capture and detection antibodies bind to different epitopes of the cytokine
  • No cross-reactivity with hIFN-β, murine (m)IFN-α2, or mIFN-β

LumiKine™ Xpress hIFN-α 2.0 is provided with a standard recombinant hIFN-α2 produced in mammalian cells, to ensure the most accurate and sensitive calibration for the measurement of unknown levels of hIFN-α.

All products are for research use only, and not for human or veterinary use.

LumiKine™ Xpress hIFN-α
LumiKine™ Xpress hIFN-α

SPECIFICATIONS

Specifications

Applications

Quantification of cytokine levels in multiple sample types

Target

IFN-α

Target species

Human

Sample types
Cell culture supernatant, serum, plasma
Sample volume

50 µl

Limit of detection

5 pg/ml

Assay time
2 hours (after plate preparation)
Detection method
Bioluminescence
Tested applications

Quantification of hIFN-α2 in serum sample

Quality control

Each lot is functionally tested and validated.

Additional information

No cross-reactivity with human IFN-β, mouse (m)IFN-α2 or mIFN‑β

CONTENTS

Contents

  • Product: 
    LumiKine™ Xpress hIFN-α 2.0
  • Cat code: 
    luex-hifnav3
  • Quantity: 
    5 plates
Includes:
  • 150 µg lyophilized hIFN-α capture antibody
  • 5 µg lyophilized Lucia-conjugated detection antibody
  • 100 ng lyophilized hIFN-α2 standard (HEK293-expressed recombinant cytokine)
  • 2 tubes of QUANTI-Luc™ 4 Reagent (sufficient to prepare 50 ml)
  • 5 white flat-bottom MaxiSorp® 96-well plates with 5 plate sealers

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: See TDS

    Caution:

    • Avoid repeated freeze-thaw cycles
    • Protect from light

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