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Jurkat-Raji PD-1/PD-L2 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human PD-1 expression by Jurkat-Lucia™ TCR-hPD-1 cells.

Validation of human PD-L1 and PD-L2 expression by Raji-APC-hPD-L2 cells

Activation of Jurkat-Lucia™ TCR-hPD-1 cells using Anti-hPD-1 biosimilar mAbs

Activation of Jurkat-Lucia™ TCR-hPD-1 cells using Anti-hPD-L1 or Anti-hPD-L2 mAbs, alone or in combination

Jurkat-Raji (PD-1/CTLA4)/PD-L1 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human CD28, CTLA4, and PD-1 expression by Jurkat-Lucia™ TCR-hCTLA4-hPD-1 cells

Validation of human CD80 and PD-L1 expression by Raji-APC-hPDL1-derived cells

Activation of Jurkat-Lucia™ TCR-hCTLA4-hPD-1 cells using Anti-hPD-1 and Anti-hCTLA4 biosimilar mAbs, alone or in combination

Jurkat-Raji CTLA4/CD80 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

Validation of human CD28 and CTLA4 overexpression by Jurkat-Lucia™ TCR-hCTLA4 cells

Validation of endogenous human CD80 expression by Raji-APC-Null cells

ation of Jurkat-Lucia™ TCR-hCTLA4 cells using Anti-hCTLA4 biosimilar mAb

Human ASC-GFP Reporter THP-1 Cells

ASC-GFP speck-forming monocytes

Visualization of ASC speck formation by fluorescence microscopy

Human STING-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Human IFI16-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IFI16 knockout

IRF INDUCTION (Lucia luciferase reporter)

Human MyD88-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of MyD88 knockout

NF-κB responses in THP1-Dual™ KO-MyD cells

Human Caspase 1-KD THP-1 Cells

CASP1 knockdown monocytes

NF-κB Reporter THP-1 Cells (Lucia®)

NF-κB-Lucia reporter monocytes

NF-κB responses of THP1-Lucia™ NF-κB cells

NF-κB Reporter THP-1 Cells (SEAP)

NF-κB-SEAP reporter monocytes

NF-κB responses in THP1-Blue™ NF-κB cells

Control Reporter THP-1 Cells - THP1-Null Cells

Control monocytes

Human NLRC4 THP-1 Cells

NLRC4 overexpressing monocytes

Inflammasome response using different Flagellins

Inflammasome response using Needle-Tox

Jurkat & Raji PD-1/PD-L1 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

CD28 and PD-1 expression on Jurkat-Lucia™ TCR-hPD-1 cells

PD-L1 expression on Raji-APC-derived cells

Disruption of PD-1/PD-L1 inhibitory interaction using Anti-hPD-1 or Anti-hPD-L1 antibodies

Human TLR7 Dual Reporter THP-1 Cell Lines

Two cell lines (TLR7-OE w/o TLR8) - NF-κB-SEAP & IRF-Lucia reporter human monocytes

NF-κB responses in TLR7 overexpressing THP1-Dual™ -derived cells

IRF responses in TLR7 overexpressing THP1-Dual™ -derived cells

Human MD2-CD14-TLR4/TLR4-KO Dual Reporter THP-1 Cell Lines

Two cell lines (TLR4-OE or TLR4-KO) - NF-κB-SEAP & IRF-Lucia reporter monocytes

Functional validation of NF-κB response

NF-κB responses after stimulation with rough or smooth LPS

Functional validation of IRF response

IRF responses after stimulation with rough or smooth LPS

PCR validation of TLR4 KO in THP1-Dual™ MD2-CD14 KO-TLR4 cells

Human MDA5-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of MDA5 knockout

Functional validation of MDA5 knockout (IRF response)

Functional validation of MDA5 knockout (NF-κB response)

Jurkat & Raji ICOS/ICOS-L Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NFAT-Lucia reporter T lymphocytes

ICOS overexpression on Jurkat-Lucia™ hICOS cells

ICOS-L overexpression on Raji-hICOS-L cells

Disruption of ICOS/ICOS-L activatory activation

Jurkat & Raji CD27/CD70 Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NF-κB-Lucia reporter T lymphocytes

CD27 expression on Jurkat-Lucia™ hCD27 cells

CD70 expression on Raji-Null cells

Disruption of CD27/CD70 activatory interaction

Jurkat & Raji 4-1BB/4-1BBL Antagonist Assay (Bio-IC™)

Two cell line bioassay - B lymphocytes & NF-κB-Lucia reporter T Lymphocytes

4-1BB expression on Jurkat-Lucia™ h4-1BB cells

4-1BBL expression on Raji-Null cells

Disruption of 4-1BB/4-1BBL activatory interaction

IKK epsilon-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter human monocytes - IKKε deficient

Validation of IKKε KO by PCR and Western blot

Functional validation of IKKε knockout (NF-κB and IRF response)

RNA-induced responses in THP1-Dual™ KO-IKKε cells