STING-HA

Expression-ready ORF Clones

HA-tagged STING gene

Alias: STING1, TMEM173, MITA, MPYS

STING is essential for the IFN response to microbial or self-DNA, and acts as a direct sensor of cyclic dinucleotides (CDNs).
CDNs are important messengers in bacteria, affecting numerous responses of the prokaryotic cell, but also in mammalian cells, acting as agonists of the innate immune response.

Several non-synonymous variants of STING have been described in the human population. The prevalent human STING isoform, which contains an arginine at position 232 (R232), is present in ~60% of the human population and therefore, is considered the wild-type [1,2]. The hSTING-WT isoform is preferentially activated by 2’5’ linkage-containing cGAMP isomers [3].

The gene has been fused at the 3' end to three influenza hemagglutinin (HA) epitope tags. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expressed genes by Western blot.

Stimulator of interferon response cGAMP interactor 1.
Stimulator of interferon genes.
Transmembrane protein 173.

References:

1. Jin L. et al., 2011. Identification and characterization of a loss-of-function human MPYS variant. Genes Immun. 12(4):263-9.
2. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect Innate immune response to cyclic dinucleotides. PLoS One 8(10):e77846.
3. Gao P. et al., 2013. Structure-function analysis of STING activation by c[G(2',5')pA(3',5')p] and targeting by antiviral DMXAA. Cell. 154(4):748-62.