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pFUSE-CHIg-hG4e1

pFUSE-CHIg-hG4e1 Unit size Cat. code Docs Qty Price
Human IgG4
20 µg
pfuse-hchg4e1
+-
$536.00
pFUSEss-CHIg-hG4e1 Unit size Cat. code Docs Qty Price
Human IgG4
20 µg
pfusess-hchg4e1
+-
$536.00

pFUSE-CHIg-hG4e1 is a cloning plasmid that expresses the human IgG4 heavy chain constant region with the S228P mutation for reduced Fab-arm exchange. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG4e1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

  • Labrijn AF. et al., 2009. Therapeutic IgG4 antibodies engage in Fab- arm exchange with endogenous human IgG4 in vivo. Nat Biotechnol. 27(8):767-71.
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Specifications

• pFUSE-CHIg-hG4e1 features the constant region of the human IgG4 heavy chain with the S228P mutation.

pFUSEss-CHIg-hG4e1 features the constant region of the human IgG4 heavy chain with the S228P mutation and the IL2 signal sequence

Characteristics of the S228P mutation:
- Reduced Fab-arm exchange

Both plasmids are selectable with Zeocin™.

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Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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