Engineered Heavy chain (CH)
Engineered pFUSE-CHIg express mutant forms of the constant regions of the heavy chain (CH) of the human IgG1. They contain a multiple cloning site (MCS) upstream of these constant regions to enable the cloning of the variable (VH) regions of a given antibody. Engineered Fc alow the production of IgG with modified effector function.
All engineered pFUSE-CHIg plasmids are also available with the IL2 signal sequence to allow the secretion of recombinant IgGs generated using Fab or scFv fragments selected from phage display libraries that lack a signal sequence.
pFUSE-CLIg and pFUSE-CHIg plasmids are designed to change a monoclonal antibody from one isotype to another or to produce entire IgG antibodies from Fab or scFv fragments.
Transfection of mammalian cell lines with the recombinant pFUSE-CHIg and pFUSE-CLIg pair allows to generate an IgG antibody that can be purified from the supernatant using the appropriate Protein A, Protein G or Protein L affinity chromatography.