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pFUSE-CHIg-hG1e4

pFUSE-CHIg-hG1e4 Unit size Cat. code Docs Qty Price
Human IgG1
20 µg
pfuse-hchg1e4
+-
$536.00
pFUSEss-CHIg-hG1e4 Unit size Cat. code Docs Qty Price
Human IgG1
20 µg
pfusess-hchg1e4
+-
$536.00

pFUSE-CHIg-hG1e4 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with the E333A mutation. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e4 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

- Shields RL. et al., 2001. High resolution mapping of the binding site on human IgG1 for Fc gamma RI, Fc gamma RII, Fc gamma RIII, and FcRn and design of IgG1 variants with improved binding to the Fc gamma R. J Biol Chem. 276(9):6591-604.
- Idusogie EE. et al., 2001. Engineered antibodies with increased activity to recruit complement. J Immunol. 166(4):2571-5.

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Specifications

• pFUSE-CHIg-hG1e4 features the constant region of the human IgG1 heavy chain with the E333A mutation

pFUSEss-CHIg-hG1e4 features the constant region of the human IgG1 heavy chain with the E333A mutation and the IL2 signal sequence

Characteristics of E333A mutation:
- Increased binding to FcγRIIIa
- Increased ADCC and CDC

Both plasmids are selectable with Zeocin™.

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Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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