Invivogen
Menu

pFUSE-CHIg-hG1e2

pFUSE-CHIg-hG1e2 Unit size Cat. code Docs Qty Price
Human IgG1
20 µg
pfuse-hchg1e2
+-
$536.00
pFUSEss-CHIg-hG1e2 Unit size Cat. code Docs Qty Price
Human IgG1
20 µg
pfusess-hchg1e2
+-
$536.00

pFUSE-CHIg-hG1e2 is a cloning plasmid that expresses the human IgG1 heavy chain constant region with M252Y / S254T / T256E + H433K / N434F mutations. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-hG1e2 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Vaccaro C. et al., 2005. Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat Biotechnol. 23(10):1283-8.

Back to the top

Specifications

• pFUSE-CHIg-hG1e2 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations

pFUSEss-CHIg-hG1e1 features the constant region of the human IgG1 heavy chain with M252Y / S254T / T256E + H433K / N434F mutations and the IL2 signal sequence

Characteristics M252Y / S254T / T256E + H433K / N434F mutations:
- Increased binding to FcRn
- Increased half-life

Both plasmids are selectable with Zeocin™.

Back to the top

Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

Back to the top

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
7 + 1 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty