Fc Effector Activity Cellular Bioassays

Engineered Jurkat-NFAT Reporter cells for ADCC and ADCP

InvivoGen’s cell-based effector activity reporter assays provide biologically relevant tools to assess antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP). They are designed to evaluate the potency and Fc-effector activity of monoclonal antibodies (mAbs) and other biologics that engage with Fc-gamma receptors (FcγR).

These bioluminescent assays are based on Jurkat-Lucia™ effector cells stably expressing a specific Fcγ receptor variant, either CD64 (FcγRI), CD32A (FcγRIIa), CD16A (FcγRIIIa) high-affinity allotype V158, or CD16A low-affinity allotype F158, together with an NFAT-inducible Lucia® luciferase reporter gene. 

Key features

• No primary cell culture required
• Robust and reproducible assay design
• Sensitive Lucia® luciferase readout
• Suitable for antibody potency testing and Fc engineering

Applications

• Screening of potent biologics for ADCC or ADCP induction
• Evaluation of Fc engineering strategies to modulate effector potency
• Testing of novel anti-CD16, -CD32, or -CD64 mAbs

Co-culture of Jurkat-Lucia™ NFAT effector cells with antigen-presenting target cells (e.g., Raji cells) in the presence of test antibodies leads to NFAT-mediated Lucia® reporter activation. The intensity of the Lucia® signal provides a direct, quantitative measure of the antibody’s Fc-mediated effector function.