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Human ACE2 & TMPRSS2 & NLRP1 & ASC-GFP Reporter A549 Cell Lines

Two cell lines (ACE2-OE & TMPRSS2-OE w/o NLRP1-OE) - ASC-GFP speck forming lung carcinoma

Overexpression of TMPRSS2 (qRT-PCR)

Cell surface expression of ACE2 (flow-cytometry)

NLRP1 and ASC-GFP expression (Western blot)

ASC speck formation upon NLRP1 inflammasome activation (with hTNF-α stimulation)

ASC speck formation upon NLRP1 inflammasome activation (without hTNF-α stimulation)

Pyroptotic cell death upon NLRP1 inflammasome activation

Human MD2-CD14-TLR4/TLR4-KO Dual Reporter THP-1 Cell Lines

Two cell lines (TLR4-OE or TLR4-KO) - NF-κB-SEAP & IRF-Lucia reporter monocytes

Functional validation of NF-κB response

NF-κB responses after stimulation with rough or smooth LPS

Functional validation of IRF response

IRF responses after stimulation with rough or smooth LPS

PCR validation of TLR4 KO in THP1-Dual™ MD2-CD14 KO-TLR4 cells

Human MDA5-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of MDA5 knockout

Functional validation of MDA5 knockout (IRF response)

Functional validation of MDA5 knockout (NF-κB response)

Human STING-KI Dual Reporter THP-1 Cell Lines

Five KI-STING variants (R232, H232, M155, S154, A162) - NF-κB-SEAP & IRF-Lucia reporter monocytes

NF-κB responses in THP1-Dual™ KI-hSTING-R232 and H232 cells

IRF responses in THP1-Dual™ KI-hSTING-R232 and H232 cells

NF-κB responses in THP1-Dual™ KI-hSTING-M155 and S154 SAVI cells

IRF responses in THP1-Dual™ KI-hSTING-M155 and S154 SAVI cells

NF-κB responses in THP1-Dual™ KI-hSTING-A162 cells

IRF responses in THP1-Dual™ KI-hSTING-A162 cells

Human TLR3 Dual Reporter THP-1 Cells

TLR3-OE - NF-κB-SEAP & IRF-Lucia reporter human monocytes

Functional validation of NF-κB response

Functional validation of IRF response

IKK epsilon-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter human monocytes - IKKε deficient

Validation of IKKε KO by PCR and Western blot

Functional validation of IKKε knockout (NF-κB and IRF response)

RNA-induced responses in THP1-Dual™ KO-IKKε cells

Human ACE2 & TMPRSS2 A549 Cell Lines

Two cell lines (ACE2-OE w/o TMPRSS2-OE) - Lung carcinoma

Validation of ACE2 overexpression by RT-qPCR

Validation of ACE2 surface expression on A549-hACE2 cells by FACS

Infection of A549-hACE2 cells by SARS-CoV-2 Spike pseudotyped lentiviral particles

Validation of ACE2 and TMPRSS2 overexpression by RT-qPCR

Validation of ACE2 surface expression on A549-hACE2-TMPRSS2 cells by FACS

Infection of A549-hACE2-TMPRSS2 cells by SARS-CoV-2 Spike pseudotyped lentiviral particles

Human NLRC4-KO THP-1 Cells

NLRC4-KO monocytes

Validation of NLRC4 Knockout (KO)

Functional validation of THP1-KO-NLRC4 cells

Human SAMHD1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of SAMHD1 knockout

Functional validation of SAMHD1 knockout (NF-κB response)

Functional validation of SAMHD1 knockout (IRF response)

Human DNase2-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of DNASE2 knockout

Functional validation of DNASE2 knockout (NF-κB response)

Functional validation of DNASE2 knockout (IRF response)

Human NLRP1 & ASC-GFP Reporter A549 Cell Lines

Two cell lines (w/o NLRP1-OE) - ASC-GFP speck forming lung carcinoma

Validation of NLRP1 and inducible ASC::GFP expression

Validation of inducible ASC::GFP expression

Monitoring of ASC speck formation and pyroptosis upon NLRP1 activation using Val-boroPro

Monitoring of ASC speck formation and pyroptosis upon NLRP1 activation using Poly(IC)

Human IRF7-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF7 KO

Functional validation of IRF7 knockout (NF-κB response)

Functional validation of IRF7 knockout (IRF response)

Human IRF5-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF5 KO

Functional validation of IRF5 knockout (NF-κB response)

Functional validation of IRF5 knockout (IRF response)

Human IRF1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF1 KO

Functional validation of IRF1 knockout (NF-κB response)

Functional validation of IRF1 knockout (IRF response)

Human Caspase 4-KO THP-1 Cells

CASP4-KO monocytes

Validation of CASP4 KO in THP1-KO-CASP4 cells

Secretion of mature IL-1β by THP1-KO-CASP4 cells

Human ASC-KO THP-1 Cells

ASC-KO monocytes

Validation of ASC KO in THP1-KO-ASC cells

Absence of mature IL-1β secretion by THP1-KO-ASC cells

Absence of pyroptosis in THP1-KO-ASC cells

Human NLRP3-KO THP-1 Cells

NLRP3-KO monocytes

Validation of NLRP3 KO in THP1-KO-NLRP3 cells

Absence of mature IL-1β secretion in THP1-KO-NLRP3 cells

Absence of pyroptosis in THP1-KO-NLRP3 cells (LDH read-out)

Mouse STING-KI Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of mSTING knockin by PCR

Validation of mSTING knockin by Western blot (WES by Protein Simple)

IRF activation (Lucia luciferase reporter)

NF-kB activation (SEAP reporter)

Human IRF3-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF3 knockout by PCR

Validation of IRF3 knockout by Western blot (Wes™)

2'3'-cGAMP-induced IRF and NF-κB responses in THP1-Dual™-derived cells

RNA-induced IRF and NF-κB responses in THP1-Dual™-derived cells

Cytokine or LPS-induced IRF and NF-κB responses in THP1-Dual™-derived cells

Human TBK1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of TBK1 knockout by Western blot (Wes™)

2’3’-cGAMP-induced IRF and NF-κB responses in THP1-Dual-derived cells

RNA-induced IRF and NF-κB responses in THP1-Dual-derived cells

Cytokine or LPS-induced IRF and NF-κB responses in THP1-Dual-derived cells