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pFUSE-mIgG1e2-Fc

pFUSE-mIgG1e2-Fc1 Unit size Cat. code Docs Qty Price
20 µg
pfc1-mg1e2
+-
$536.00
pFUSE-mIgG1e2-Fc2 Unit size Cat. code Docs Qty Price
20 µg
pfc2-mg1e2
+-
$536.00

pFUSE-mIgG1e2-Fc1 is a cloning plasmid for the generation of Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG1 heavy chain with the T252M mutation and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.

pFUSE-mIgG1e2-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.

Nagaoka M. & Akaike T., 2003 Single amino acid substitution in the mouse IgG1 Fc region induces drastic enhancement of the affinity to protein A. Protein Eng. 16(4):243:245. 

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Specifications

Isotype : IgG1 mouse
Mutation
: T252M
Characteristics:

- Increased protein A affinity
- Reduced CDC, no ADCC

Both plasmids are selectable with Zeocin™.

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Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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