pFUSE-mIgG1-Fc

pFUSE-mIgG1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the murine IgG1 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. Murine IgG1 displays low ADCC and CDC.

pFUSE-mIgG1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.

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Specifications

Isotype : IgG1 mouse
Effector Activities : ADCC-, CDC +/-
Protein A binding: +
Protein G binding: ++++

Both plasmids are selectable with Zeocin™.

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Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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