pFUSE-hIgG1e1-Fc

pFUSE-hIgG1e1-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG1 heavy chain mutated T250Q/M428L and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.

pFUSE-hIgG1e1-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.

Mutations: T250Q/M428L
Characteristics:
- Increased binding to neonatal FcR (FcRn)
- Increased half-life

Reference

Hinton PR. et al., 2004. Engineered human IgG antibodies with longer serum half-lives in primates. J Biol Chem. 279(8):6213-6.