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pFUSE-hIgG1e2-Fc

pFUSE-hIgG1e2-Fc1 Unit size Cat. code Docs Qty Price
20 µg
pfc1-hg1e2
+-
$536.00
pFUSE-hIgG1e2-Fc2 Unit size Cat. code Docs Qty Price
20 µg
pfc2-hg1e2
+-
$536.00

pFUSE-hIgG1e2-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. The engineered hIgG1e2 Fc contains the following mutations: M252Y/S254T/T256E and H433K/N434F.

pFUSE-hIgG1e2-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.

Vaccaro C. et al., 2005. Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat Biotechnol. 23(10):1283-8.

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Specifications

Isotype: IgG1 human
Mutations: M252Y/S254T/T256E + H433K/N434F
Characteristics:
- Increased binding to neonatal FcR (FcRn)
- Increased half-life

Both plasmids are selectable with Zeocin™.

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Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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