pDRIVE-mIFN-β
| pDRIVE-mIFNB | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pDRIVE bearing mouse IFN-β promoter - LacZ |
20 µg |
pdrive-mifnb |
| pDRIVE5SEAP-mIFNB | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pDRIVE bearing mouse IFN-β promoter - SEAP |
20 µg |
pdrive5s-mifnb |
| pDRIVE5Lucia-mIFNB | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
pDRIVE bearing mouse IFN-β promoter - Lucia |
20 µg |
pdrive5lc-mifnb |
Mouse INF-β promoter (Hematopoietic cells) in pDRIVE expression plasmid
Expression of the interferon-beta (IFN-β) gene is induced by a variety of agents, including viruses and double-stranded RNA (dsRNA), and is also strongly repressed in uninduced cells, a requirement imposed by the cytotoxic properties of IFN-β.
The functional IFN-β promoter resides within a 170 bp fragment located 5’ of the coding sequence [1].
This region contains four binding sites for transcription factors such as NF-κB and IRF-1 that act as activators, as well as putative negative regulatory domains.
Fusion of the IFN-β promoter region to the luciferase gene was shown to render the expression of the reporter gene inducible by dsRNA [2].
References:
1. Dirks W. et al., 1989. Isolation and functional characterization of the murine interferon-beta 1 promoter. J Interferon Res. 9(1):125-33.
2. King P& Goodbourn S. 1994. The betainterferon promoter responds to priming through multiple independent regulatory elements. J Biol Chem. 269(48):30609-15.
Specifications
pDRIVE bearing mouse IFN-β promoter - LacZ, SEAP or Lucia
Gene Promoter: Interferon Beta
Specificity: Hematopoietic cells
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
Back to the toppDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
