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pDRIVE-mIFN-β

Product Unit size Cat. code Docs. Qty. Price

pDRIVE-mIFNB

pDRIVE bearing mouse IFN-β promoter - LacZ

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20 µg

pdrive-mifnb
+-
$554

pDRIVE5SEAP-mIFNB

pDRIVE bearing mouse IFN-β promoter - SEAP

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20 µg

pdrive5s-mifnb
+-
$554

pDRIVE5Lucia-mIFNB

pDRIVE bearing mouse IFN-β promoter - Lucia

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20 µg

pdrive5lc-mifnb
+-
$554

Mouse IFN-β promoter (Hematopoietic cells) in pDRIVE expression plasmid

Expression of the interferon-beta (IFN-β) gene is induced by a variety of agents, including viruses and double-stranded RNA (dsRNA), and is also strongly repressed in uninduced cells, a requirement imposed by the cytotoxic properties of IFN-β.

The functional IFN-β promoter resides within a 170 bp fragment located 5’ of the coding sequence [1].

This region contains four binding sites for transcription factors such as NF-κB and IRF-1 that act as activators, as well as putative negative regulatory domains.

Fusion of the IFN-β promoter region to the luciferase gene was shown to render the expression of the reporter gene inducible by dsRNA [2].

 

References:

1. Dirks W. et al., 1989. Isolation and functional characterization of the murine interferon-beta 1 promoter. J Interferon Res. 9(1):125-33.
2. King P& Goodbourn S. 1994. The betainterferon promoter responds to priming through multiple independent regulatory elements. J Biol Chem. 269(48):30609-15.

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Specifications

pDRIVE bearing mouse IFN-β promoter - LacZ, SEAP or Lucia

Gene Promoter: Interferon Beta

Specificity: Hematopoietic cells

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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