Recombinant human IL-5 protein - Bioactive cytokine

Recombinant cytokine, source: CHO cells

ABOUT

Human IL-5 protein - Mammalian cell-expressed, tag-free, carrier-free

Recombinant human IL-5 is a high-quality and biologically active cytokine, validated using proprietary IL-5 reporter cells. This member of the common β-chain (βc) receptor cytokine family is produced in CHO cells to ensure protein glycosylation and bona fide 3D structure.

Recombinant human IL-5 can be used together with HEK-Blue™ IL-5 cells for the screening of inhibitory molecules, such as Benralizumab, a therapeutic monoclonal antibody blocking IL-5 signaling (see figure).

 

Key features

  • Each lot is validated using HEK-Blue™ IL-5 cells
  • Endotoxin ≤ 0.05 EU/µg
  • 0.2 µm sterile-filtered

Applications

  • Standard for IL-5 detection and quantification
  • Screening and release assays for antibodies blocking IL-5 signaling
  • Screening and release assays for engineered IL-5

 

Interleukin 5 (IL-5) is a secreted pro-inflammatory cytokine that specifically induces the differentiation of eosinophils, which play important roles in host defense as well as in pathologies, including allergies.

More details

All products are for research use only, and not for human or veterinary use.

IL-5 signaling and biological functions
IL-5 signaling and biological functions

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SPECIFICATIONS

Specifications

Source
CHO cells
Species
Human
Synonyms
B cell differentiation factor I
Eosinophil differentiation factor
EDF
T cell replacing factor
TRF
Accession sequence

P05113

Protein size
115 a.a. (I20 - S134)
Molecular weight
~ 16 kDa (SDS-PAGE)
Carrier
Carrier-free
Tag
Tag-free
Purity
≥ 95% (SDS-PAGE)
Solubility

100 μg/ml in water

Formulation buffer

Phosphate buffer saline (pH 7.4), saccharose

Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (provided)
Sterility

0.2 µm filtration

Endotoxin

The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Applications

Cellular assays (tested)

ELISA

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Recombinant human IL-5
  • Cat code: 
    rcyc-hil5
  • Quantity: 
    10 µg
Includes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20 °C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Interleukin 5 (IL-5) is a secreted pro-inflammatory cytokine that specifically induces the differentiation of eosinophils, which play important roles in host defense as well as in pathologies, including allergies [1]. It also stimulates B cell growth and increases immunoglobulin secretion. Homodimeric IL-5 signals through the heterodimeric cell surface IL-5 receptor (IL‑5R) consisting of IL-5Rα (also called CD125) and IL-5RB, (also called the cytokine receptor common subunit beta, CSF2RB or CD131). The binding of IL-5 to its receptor triggers three main signaling pathways: JAK/STAT, PI3K, and MAPK/ERK [1-2]. Of note, monomeric IL-5 has no activity [2]. The pivotal role of IL-5 in eosinophil differentiation makes it an attractive target in eosinophilic conditions, such as asthma [3]. 

 

References 

1. Dougan M. et al., 2019. GM-CSF, IL-3, and IL-5 family of cytokines: regulators of inflammation. Immunity. 50(4):796-811. 
2. Morris R. et al., 2018. The molecular details of cytokine signaling via the JAK/STAT pathway. Protein Sci. 27(12):1984-2009. 
3. Yanagibashi T. et al., 2017. Allergic diseases: From bench to clinic - Contribution of the discovery of interleukin-5. Cytokine. 98:59-70.

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