CLR Reporter Cells
Dectin-1 is a member of the C-type lectin receptor (CLR) family and plays an important role in antifungal innate immunity. Upon binding to its ligand, Dectin-1 triggers phagocytosis and signaling through the kinase Syk and the adaptors CARD9-Bcl10-Malt1 leading to the production of reactive oxygen species (ROS), the activation of NF-kB and the subsequent production of pro-inflammatory cytokines. Dectin-1 signaling has been shown to collaborate with Toll-like receptor 2 (TLR2) signaling to enhance the responses triggered by each receptor.
Dectin-2 also plays an important role in antifungal innate immunity. This receptor binds to mannose carbohydrates, such as the mannose-capped lipoarabinomannan of mycobacteria and α-mannans found in Candida albicans. Upon binding to its ligand, Dectin-2 couples with the Fc receptor gamma chain and signals through the kinase Syk and the adaptors CARD9/Bcl-10/MALT1, triggering the activation of NF-κB and the subsequent production of pro-inflammatory cytokines.
Mincle is a multi-task danger receptor that recognizes a wide variety of ligands such as damaged cells, fungus, yeast and mycobacteria. It detects trehalose-6,6-dibehenate (TDB) and heat-killed M. tuberculosis (HKMT).
InvivoGen offers several SEAP reporter cell lines that respond to CLR ligands : a macrophage derived CLR reporter cell line, and several HEK-Blue™ reporter cell lines, engineered from human HEK293, that stably express a given CLR gene