Adilipoline™ (CL413) - TLR2 & TLR7 Bispecific Agonist

Chimeric molecule

ABOUT

Bispecific TLR2 & TLR7 ligand

Adilipoline™ (S-(2,3-bis(palmitoyloxy)- (2RS)propyl)- (R)-cysteinyl-(S)-seryl-(S)- lysyl- (S)-lysyl- (S)-lysyl- (S)-lysyl 4 -((6-amino-2- (butylamino)-8- hydroxy-9H- purin-9-yl) methyl) aniline) was generated by conjugation of an 8-hydroxyadenine moiety to the terminal acid function of Pam2CSK4, a TLR2 agonist.

Adilipoline™ is a good ligand for both TLR2 and TLR7. In vivo tumor studies have demonstrated that Adilipoline™ is a potent antitumor agent. Intratumoral injection of Adilipoline™ in established B16 tumors resulted in tumor regression.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Synonyms
S-(2,3-bis (palmitoyloxy)-(2RS) propyl)- (R)-cysteinyl- (S)-seryl- (S)-lysyl- (S)-lysyl- (S)-lysyl-(S)- lysyl 4-((6-amino-2- (butylamino)-8-hydroxy- 9H-purin-9-yl)methyl) aniline
Chemical formula

C81H145N17O12S

Molecular weight
1581 g/mol
Working concentration

50 pg- 10 µg/ml (~30 pM - 10 µM)

Purity
≥95% (UHPLC)
Solubility

H2O (1 mg/ml)

Appearance (form)
Lyophilized powder
Tested applications

Activation of TLR2 and TLR7 in cellular assays

Quality control

Each  lot is functionally tested and validated

CONTENTS

Contents

  • Product: 
    Adilipoline™ (CL413)
  • Cat code: 
    tlrl-c413
  • Quantity: 
    500 µg
Includes:

1.5 ml of endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • 4°C
    Stability: 6 months at 4°C

    Caution:

    • Avoid repeated freeze-thaw cycles
    • Do not store in plastic tubes

Details

HEK-Blue™ hTLR2 cells stimulation

HEK-Blue™ hTLR2 cells  which stably express an NF-κB-inducible SEAP reporter gene and human TLR2 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

HEK-Blue™ hTLR7 cells stimulation

HEK-Blue™ hTLR7 cells, which stably express an NF-κB-inducible SEAP reporter gene and human TLR7 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

RAW-Blue™ cells, which stably express an NF-kB-inducible SEAP reporter gene, were stimulated with 0.6 mg/ml of multi-PRR ligands

RAW-Blue™ cells, which stably express an NF-κB-inducible SEAP reporter gene, were stimulated with 0.6 µg/ml of InvivoGen’s multi-PRR ligands complexed with 0.1 µg/ml HSV-60 (synthetic dsDNA).
After 24h incubation, the levels of NF-κB-induced SEAP were determined using QUANTI-Blue™, a SEAP detection reagent.

Antitumor effect of CL401 & CL413

(A) Tumor growth after CL413 or CL401 treatment. C57BL/6 mice were inoculated subcutaneously with 5x10e5 B16-F1 mouse melanoma cells. After seven days, Adilipoline™ (CL413), CL401 or vehicle were injected intratumorally (50 µg/mouse/50 µl) on days 5, 15 and 20. (B) Survival curves for untreated as well as CL413- or CL401-treated mice.

Each group contained 8 mice. Black arrows represent the days of injection. Tumor growth was monitored and measured with calipers after day 5 of grafting tumor cells into mice and then every 2 days thereafter. Tumor volume in mm3 was determined according to the formula V = W2 x L/2, where L = length (mm) and W = width (mm).

DOCUMENTS

Documents

Adilipoline™ (CL413)

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

Need a CoA ?

CUSTOMER SERVICE & TECHNICAL SUPPORT

Question about this product ?