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Adilipoline™ (CL413)

Adilipoline™ (CL413) Unit size Cat. code Docs Qty Price
TLR2 & TLR7 Ligand
500 µg
tlrl-c413
+-
$157.00

TLR2 & TLR7 Ligand

Adilipoline™ (S-(2,3-bis(palmitoyloxy)- (2RS)propyl)- (R)-cysteinyl-(S)-seryl-(S)- lysyl- (S)-lysyl- (S)-lysyl- (S)-lysyl 4 -((6-amino-2- (butylamino)-8- hydroxy-9H- purin-9-yl) methyl) aniline) was generated by conjugation of an 8-hydroxyadenine moiety to the terminal acid function of Pam2CSK4.

Adilipoline™ is a good ligand for both TLR2 and TLR7. In vivo tumor studies have demonstrated that Adilipoline™ is a potent antitumor agent. Intratumoral injection of Adilipoline™ in established B16 tumors resulted in tumor regression. However, in contrast to AdiFectin™ (CL347), no protection after tumor rechallenge was observed.

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Specifications

Specificity: TLR2 and TLR7 agonist

Synonym: S-(2,3-bis (palmitoyloxy)-(2RS) propyl)- (R)-cysteinyl- (S)-seryl- (S)-lysyl- (S)-lysyl- (S)-lysyl-(S)- lysyl 4-((6-amino-2- (butylamino)-8-hydroxy- 9H-purin-9-yl)methyl) aniline

Working concentration: 50 pg- 10 µg/ml (~30pM - 10 µM)

Solubility: H2O (1 mg/ml)

Formula: C81H145N17O12S

Molecular weight: 1581 g/mol

Endotoxin level: <0.001 EU/µg

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Contents

  • 500 µg Adilipoline™ (CL413) provided as a lyophilized powder
  • 1.5 ml endotoxin-free water

room temperature Products are shipped at room temperature.

store Stored at -20°C.

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Description

Adilipoline chemical structure

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Details

HEK-Blue™ hTLR2 cells stimulation

HEK-Blue™ hTLR2 cells  which stably express an NF-κB-inducible SEAP reporter gene and human TLR2 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

HEK-Blue™ hTLR7 cells stimulation

HEK-Blue™ hTLR7 cells, which stably express an NF-κB-inducible SEAP reporter gene and human TLR7 were incubated in HEK-Blue™ Detection (a SEAP detection growth medium) and stimulated with increasing concentrations of the agonists indicated in the graph.
After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

RAW-Blue™ cells, which stably express an NF-kB-inducible SEAP reporter gene, were stimulated with 0.6 mg/ml of multi-PRR ligands

RAW-Blue™ cells, which stably express an NF-κB-inducible SEAP reporter gene, were stimulated with 0.6 µg/ml of InvivoGen’s multi-PRR ligands complexed with 0.1 µg/ml HSV-60 (synthetic dsDNA).
After 24h incubation, the levels of NF-κB-induced SEAP were determined using QUANTI-Blue™, a SEAP detection reagent.

Antitumor effect of CL401 & CL413

(A) Tumor growth after CL413 or CL401 treatment. C57BL/6 mice were inoculated subcutaneously with 5x10e5 B16-F1 mouse melanoma cells. After seven days, CL413, CL401 or vehicle were injected intratumorally (50 µg/mouse/50 µl) on days 5, 15 and 20. (B) Survival curves for untreated as well as CL413- or CL401-treated mice.

Each group contained 8 mice. Black arrows represent the days of injection. Tumor growth was monitored and measured with calipers after day 5 of grafting tumor cells into mice and then every 2 days thereafter. Tumor volume in mm3 was determined according to the formula V = W2 x L/2, where L = length (mm) and W = width (mm).

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