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Human TLR3 Dual Reporter THP-1 Cells

TLR3-OE - NF-κB-SEAP & IRF-Lucia reporter human monocytes

Functional validation of NF-κB response

Functional validation of IRF response

IKK epsilon-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter human monocytes - IKKε deficient

Validation of IKKε KO by PCR and Western blot

Functional validation of IKKε knockout (NF-κB and IRF response)

RNA-induced responses in THP1-Dual™ KO-IKKε cells

Human TRIF-KO Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

Validation of TRIF KO

IRF responses in PMA-differentiated THP1-Dual™ KO-TRIF cells

NF-κB responses in PMA-differentiated THP1-Dual™ KO-TRIF cells

Human NLRC4-KO THP-1 Cells

NLRC4-KO monocytes

Validation of NLRC4 Knockout (KO)

Functional validation of THP1-KO-NLRC4 cells

Human SAMHD1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of SAMHD1 knockout

Functional validation of SAMHD1 knockout (NF-κB response)

Functional validation of SAMHD1 knockout (IRF response)

Human DNase2-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of DNASE2 knockout

Functional validation of DNASE2 knockout (NF-κB response)

Functional validation of DNASE2 knockout (IRF response)

Human IRF7-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF7 KO

Functional validation of IRF7 knockout (NF-κB response)

Functional validation of IRF7 knockout (IRF response)

Human IRF5-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF5 KO

Functional validation of IRF5 knockout (NF-κB response)

Functional validation of IRF5 knockout (IRF response)

Human IRF1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF1 KO

Functional validation of IRF1 knockout (NF-κB response)

Functional validation of IRF1 knockout (IRF response)

Human RIG-I-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of RIG-I knockout

Functional validation of RIG-I knockout (IRF response)

Functional validation of RIG-I knockout (NF-κB response)

Human Caspase 4-KO THP-1 Cells

CASP4-KO monocytes

Validation of CASP4 KO in THP1-KO-CASP4 cells

Secretion of mature IL-1β by THP1-KO-CASP4 cells

Human ASC-KO THP-1 Cells

ASC-KO monocytes

Validation of ASC KO in THP1-KO-ASC cells

Absence of mature IL-1β secretion by THP1-KO-ASC cells

Absence of pyroptosis in THP1-KO-ASC cells

Human NLRP3-KO THP-1 Cells

NLRP3-KO monocytes

Validation of NLRP3 KO in THP1-KO-NLRP3 cells

Absence of mature IL-1β secretion in THP1-KO-NLRP3 cells

Absence of pyroptosis in THP1-KO-NLRP3 cells (LDH read-out)

Mouse STING-KI Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of mSTING knockin by PCR

Validation of mSTING knockin by Western blot (WES by Protein Simple)

IRF activation (Lucia luciferase reporter)

NF-kB activation (SEAP reporter)

Human IRF3-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of IRF3 knockout by PCR

Validation of IRF3 knockout by Western blot (Wes™)

2'3'-cGAMP-induced IRF and NF-κB responses in THP1-Dual™-derived cells

RNA-induced IRF and NF-κB responses in THP1-Dual™-derived cells

Cytokine or LPS-induced IRF and NF-κB responses in THP1-Dual™-derived cells

Human TBK1-KO Dual Reporter THP-1 Cells

NF-κB-SEAP & IRF-Lucia reporter monocytes

Validation of TBK1 knockout by Western blot (Wes™)

2’3’-cGAMP-induced IRF and NF-κB responses in THP1-Dual-derived cells

RNA-induced IRF and NF-κB responses in THP1-Dual-derived cells

Cytokine or LPS-induced IRF and NF-κB responses in THP1-Dual-derived cells

Human TLR4-KO Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

PCR validation of TLR4 KO

NF-κB responses in THP1-Dual™ KO-TLR4 cells

IRF responses in PMA-differentiated THP1-Dual™ KO-TLR4 cells

Human TLR2-KO Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

Validation of TLR2 KO

NF-κB responses in THP1-Dual™ KO-TLR2 cells

IRF responses in THP1-Dual™ KO-TLR2 cells

Human TLR9 Dual Reporter THP-1 Cells

NF-κB-SEAP and IRF-Lucia reporter monocytes

Validation of TLR9 overexpression

Validation of the NF-κB and IRF reporter systems in THP1-Dual™ hTLR9 cells

NF-κB and IRF responses to TLR9 agonists in THP1-Dual™ hTLR9 cells

Human LC3B Reporter THP-1 Cells

RFP::GFP::LC3-OE reporter monocytes

Detection of autophagic fluorescent puncta