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TLR09-DN

pUNO1-hTLR09a-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TLR9a-DN gene
20 µg
puno1-htlr9a-dn
+-
$406.00
pUNO1-mTLR09-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TLR9-DN gene
20 µg
puno1-mtlr9-dn
+-
$406.00
pUNO1-hTLR09a-DN-HA Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TLR9-DN gene, with HA-tag
20 µg
puno1ha-htlr9a-dn
+-
$406.00
pUNO1-mTLR09-DN-HA Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TLR9-DN gene, with HA-tag
20 µg
puno1ha-mtlr9-dn
+-
$406.00

Expression-ready ORF Clones

Alias : TLR9, CD289

TLR9-DN (ΔTIR) : Dominant Negative TIR-less TLR9 gene

 

TLR9, which is localized intracellularly, is involved in the recognition of specific unmethylated CpG-ODN sequences, that distinguishes bacterial DNA from mammalian DNA. TLR9 engages an intracellular pathway that involves MyD88 leading to NF-κB translocation [1]. TLR9 has been shown to recognize different CpG motifs; the optimal sequences being GTCGTT and GACGTT for hTLR9 and mTLR9 respectively [2]. The ectodomains of hTLR9 and mTLR9 present distinct distribution patterns of leucine-rich repeats. The sequence differences observed in the ectodomains of h- and m-TLR9 may account for the differences in ligand specificity in human versus murine TLR9.
TLR9-DN is a TIR-less form of the TLR9 generated by deleting the TIR domain (489 bp). This truncated TLR9 gene is still able to recognize its ligands but is unable to induce the signaling cascade.

1. Chuang TH. et al. (2002). J Leukoc Biol, 71(3):538-44.
2. Bauer S. et al. (2001). Proc Natl Acad Sci U S A, 98(16):9237-42.

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Specifications

Human TLR9-DN (pUNO1-hTLR09a-DN)

ORF size : 2610 bp
Subclone : MluI - NheI

Mouse TLR9-DN (pUNO1-mTLR09-DN)

ORF size : 2610 bp
Subclone : AgeI - NheI

Human TLR9-DN-HA (pUNO1-hTLR09a-DN-HA)

Subclone : MluI - NheI

Mouse TLR9-DN-HA (pUNO1-mTLR09-DN-HA)

ORF size : 2643 bp
Subclone : AgeI - NheI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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