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TLR05-DN

pUNO1-hTLR05-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TLR5-DN gene
20 µg
puno1-htlr5-dn
+-
$406.00
pUNO1-mTLR05-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TLR5-DN gene
20 µg
puno1-mtlr5-dn
+-
$406.00
pUNO1-hTLR05-DN-HA Unit size Cat. code Docs Qty Price
pUNO1 bearing the human TLR5-DN gene, with HA-tag
20 µg
puno1ha-htlr5-dn
+-
$406.00
pUNO1-mTLR05-DN-HA Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse TLR5-DN gene, with HA-tag
20 µg
puno1ha-mtlr5-dn
+-
$406.00

Expression-ready ORF Clones

Alias : TLR5, CD285

TLR5-DN (ΔTIR) : Dominant Negative TIR-less TLR5 gene

 

TLR5 is the Toll-like molecule that recognizes flagellin from both Gram+ and Gram- bacteria. It was identified by the presence of the TIR domain and is expressed in spleen, PBL and epithelial cells. Activation of the receptor stimulates the production of proinflammatory cytokines, such as TNF-α, through signaling via the adaptor protein MyD88 and the serine kinase IRAK [1, 2]. TLR5 can generate a proinflammatory signal as a homodimer suggesting that it might be the only TLR required for flagellin recognition [2]. Recent data suggest that TLR5 forms heteromeric complexes with TLR4 in macrophages in response to flagellin induction [3].
TLR5-DN is a TIR-less form of the TLR5 gene generated by deleting the TIR domain (501 bp). This truncated TLR5 gene is still able to recognize its ligands but is unable to induce the signaling cascade.

1. Gewirtz AT. et al. (2001). J Immunol, 167(4):1882-5.
2. Hayashi F. et al. (2001). Nature, 410(6832):1099-103.
3. Mizel SB. et al. (2203). J Immunol. 170(12):6217-23
.

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Specifications

Human TLR5-DN (pUNO1-hTLR05-DN)

ORF size : 2076 bp
Subclone : NcoI - NheI

Mouse TLR5-DN (pUNO1-mTLR05-DN)

ORF size : 2082 bp
Subclone : NcoI - NheI

Human TLR5-DN-HA (pUNO1-hTLR05-DN-HA)

ORF size : 2109 bp
Subclone : NcoI - NheI

Mouse TLR5-DN-HA (pUNO1-mTLR05-DN-HA)

Subclone : NcoI - NheI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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