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TICAM1

Product Unit size Cat. code Docs. Qty. Price

pUNO2-hTICAM1

pUNO2 bearing the human TICAM1 (TRIF) gene

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20 µg

puno2-hticam1
+-
$473

pUNO1-hTICAM1-DN

pUNO1 bearing a dominant negative version of the human TICAM1 (TRIF) gene

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20 µg

puno1-hticam1-dn
+-
$473

pUNO1-hTICAM1-sa

pUNO1 bearing the human saTICAM1 (super-activated TICAM1) gene

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20 µg

puno1-hticam1-sa
+-
$473

pUNO1-mTICAM1

pUNO1 bearing the mouse TICAM1 (TRIF) gene

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20 µg

puno1-mticam1
+-
$473
  • About
  • Specifications
  • Contents

Expression-ready ORF Clones

Alias : TRIF

 

Toll like receptor adaptor molecule 1.
TIR domain-containing adapter inducing IFNbeta.
TIR-containing adaptor molecule 1.

 

Available versions:

  • Wild type TICAM1
  • Dominant negative TICAM1

TICAM1 (TRIF) is an adapter involved in the MyD88-independent signaling pathway of several TLRs. A dominant-negative form of TICAM1 was shown to completely abolish TLR3-induced signaling and also to block the NF-kB activity mediated by TLR2, TLR4 and TLR7 [1]. Further analysis has revealed that TICAM1 binds preferentially to TLR3 and TLR4 and is essential in their signaling pathways [2]. It predominantly activates IRF-3 and the production of IFN-b rather than NF-kB [3].

TICAM1-DN

Dominant negative TICAM1 (aa387-566) harbors only the TIR domain and lacks the N-terminal and C-terminal portions [1]. Furthermore, it contains a proline to histidine mutation at amino acid 434. This proline residue is conserved among TLRs and is essential for activation of the TLR-mediated signaling.

1. Yamamoto M. et al. 2002. Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-b promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672
2. Yamamoto M. et al., 2003. Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science. 301(5633):640-3.
3. Oshiumi H. et al., 2003. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction.Nat Immunol. 4(2):161-7.

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Specifications

Human TICAM1 (pUNO2-hTICAM1)

ORF size : 2139 bp
Subclone : BspHI - NheI

Human TICAM1-DN (pUNO1-hTICAM1-DN)

ORF size : 546 bp
Subclone : NcoI - NheI

Human super-activated TICAM1 (pUNO1-hTICAM1-sa)

ORF size : 1935 bp
Subclone : BspHI - NheI

Mouse TICAM1 (pUNO1-mTICAM1)

Genbank : NM_174989.4
ORF size : 2199 bp
Subclone : AgeI - EcoRI

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Contents

pUNO1-mcs:

  • 20 µg of lyophilized DNA
  • 2 x 1 ml of blasticidin at 10 mg/ml

 

pUNO2-mcs:

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ at 100 mg/ml

 

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