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PYCARD

Product Unit size Cat. code Docs. Qty. Price

pUNO1-hPYCARDa

pUNO1 bearing the human PYCARD (ASC) gene, isoform 1 (long)

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20 µg

puno1-hpycarda
+-
$473

pUNO1-hPYCARD-DN

pUNO1 bearing a dominant negative version of the human PYCARD (ASC) gene

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20 µg

puno1-hpycard-dn
+-
$501

pUNO1-mPYCARD

pUNO1 bearing the mouse PYCARD (ASC) gene

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20 µg

puno1-mpycard
+-
$473

pUNO1-mPYCARD-DN

pUNO1 bearing a dominant negative version of the mouse PYCARD (ASC) gene

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20 µg

puno1-mpycard-dn
+-
$501
  • About
  • Specifications
  • pUNO1 Contents
  • Details

Expression-ready ORF Clones

Alias: ASC, CARD5, TMS1

 

PYD and CARD domain containing.
Apoptosis-associated speck-like protein containing a CARD.

 

Available versions:

  • Wild type PYCARD
  • Dominant negative PYCARD (see the "Details" tab)
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Specifications

Human PYCARD isoform 1 (long) (pUNO1-hPYCARDa)

Genbank : NM_013258.4
ORF size : 588 bp
Subclone : AgeI - NheI

Human PYCARD-DN (pUNO1-hPYCARD-DN)

ORF size : 285 bp
Subclone : AgeI - NheI

Mouse PYCARD (pUNO1-mPYCARD)

Genbank : NM_023258.4
ORF size : 582 bp
Subclone : NcoI - NheI

Mouse PYCARD-DN (pUNO1-mPYCARD-DN)

ORF size : 282 bp
Subclone : NcoI - NheI

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pUNO1 Contents

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

stability Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Alert Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.

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Details

PYCARD-DN version

The dominant negative version of PYCARD (ASC) is a truncated form of the gene (aa1-94) that contains the Pyrin domain but not the CARD domain. PYCARD-DN binds to NALP1 but not caspase-1 and strongly blocks maturation of IL-1β in PMA-differentiated THP1 cells stimulated with LPS without affecting LPS-induced proIL-1β synthesis [1].

1. Martinon F et al., 2002. The Inflammasome: A Molecular Platform Triggering Activation of Inflammatory Caspases and Processing of proIL-1β. Mol Cell (10): 417-426.

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