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MyD88

pUNO1-hMYD88 Unit size Cat. code Docs Qty Price
pUNO1 bearing the human MyD88 gene
20 µg
puno1-hmyd88
+-
$384.00
pUNO1-hMyD88-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human MyD88 gene
20 µg
puno1-hmyd88-dn
+-
$406.00
pUNO1-mMYD88 Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse MyD88 gene
20 µg
puno1-mmyd88
+-
$384.00
pUNO1-mMyD88-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the mouse MyD88 gene
20 µg
puno1-mmyd88-dn
+-
$406.00

Expression-ready ORF Clones

Myeloid Differentiation Primary Response  88 

Available versions:

  • Wild type MyD88
  • Dominant negative MyD88 (see the "Details" tab)

Myeloid differentiation factor 88 (MyD88) is a cytoplasmic adaptor protein that is involved in interleukin-1 receptor (IL-1R)- and Toll-like receptor (TLR)-induced activation of NF-kappaB. MyD88 contains a C-terminal Toll/IL-1R homology (TIR) domain and an N-terminal death domain (DD), which mediate the interaction of MyD88 with IL-1R/TLR and IRAK, respectively [1]. MyD88 is essential for signaling as MyD88 -/- mice fail to induce cytokines in response to LPS and CpG DNA [2]. Some TLRs induce antiviral responses by producing IFN-alpha in a MyD88-dependent manner. MyD88 interacts with IRF7 resulting in the activation of IFN-alpha-dependent promoters [3].
 

1. Janssens S. et al., 2002. Regulation of interleukin-1- and lipopolysaccharide-induced NF-kappaB activation by alternative splicing of MyD88. Curr Biol. 12(6):467-71.
2. Kawai T. et al. 1999. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 11(1):115-22.
3. Kawai T. et al., 2004. Interferon-alpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6. Nat Immunol. 5(10):1061-8. 

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Specifications

Human MyD88 (pUNO1-hMYD88)

Genbank : NM_002468.3
ORF size : 891 bp
Subclone : NcoI - NheI

Human MyD88-DN (pUNO1-hMyD88-DN)

ORF size : 420 bp
Subclone : NcoI - NheI

Mouse MyD88 (pUNO1-mMYD88)

ORF size : 891 bp
Subclone : BspLU11I - NheI

Mouse MyD88-DN (pUNO1-mMyD88-DN)

ORF size : 420 bp
Subclone : NcoI - NheI

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Contents

- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)
- 1 ml blasticidin at 10 mg/ml

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Details

MyD88-DN

Dominant negative MyD88 (aa161-296) is a truncated form of MyD88 that contains the C-terminal TIR domain but lacks the death domain. This MyD88-DN is unable to homodimerize and subsequently activates the signaling cascade.

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Citations

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