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MD2

pUNO1-bMD2 Unit size Cat. code Docs Qty Price
pUNO1 bearing the bovine MD2 gene
20 µg
puno1-bmd2
+-
$384.00
pUNO1-hMD2a Unit size Cat. code Docs Qty Price
pUNO1 bearing the human MD2 gene, isoform 1
20 µg
puno1-hmd2a
+-
$384.00
pUNO1-hMD2b Unit size Cat. code Docs Qty Price
pUNO1 bearing the human MD2 gene, isoform 2
20 µg
puno1-hmd2b
+-
$384.00
pUNO1-mMD2a Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse MD2 gene, isoform 1
20 µg
puno1-mmd2a
+-
$384.00

Expression-ready ORF Clones

Alias : Ly96

 

Lymphocyte antigen 96.
Myeloid differentiation factor 2.

 

MD-2, a 25-kDa secreted protein, is an accessory molecule required for efficient LPS-induced signaling through TLR4. MD-2 binds to both LPS and the extracellular domain of TLR4. Following synthesis, MD-2 is either secreted directly into the medium as a soluble, active protein, or binds directly to TLR4 in the endoplasmic reticulum before migrating to the cell surface. The secreted form of MD-2 forms a stable complex with LPS, in a CD14-dependent process [2]. The MD-2/LPS complex activates TLR4 in the absence of CD14 or free LPS. indicating that the activating ligand of TLR4 is the MD-2/LPS complex. MD-2 also increases TLR2 responsiveness to TLR2 ligands [3].

 

1. Shimazu R. et al., 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med, 189(11):1777-82.
2. Kennedy MN. et al., 2004. A complex of soluble MD-2 and lipopolysaccharide serves as an activating ligand for Toll-like receptor 4. J Biol Chem. 2004 Aug 13;279(33):34698-704.
3. Dziarski R. et al., 2001. MD-2 enables Toll-like receptor 2 (TLR2)-mediated responses to lipopolysaccharide and enhances TLR2-mediated responses to Gram-positive and Gram-negative bacteria and their cell wall components. J Immunol, 166(3):1938-44.

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Specifications

Bovine MD2 (pUNO1-bMD2)

Genbank : NM_001046517.1
ORF size : 483 bp
Subclone : BspLU11I - NheI

Human MD2 isoform 1 (pUNO1-hMD2a)

Genbank : NM_015364.4
ORF size : 483 bp
Subclone : BspLU11I - NheI

Human MD2 isoform 2 (pUNO1-hMD2b)

Genbank : NM_001159711.1
ORF size : 393 bp
Subclone : BspLU11I - NheI

Mouse MD2 isoform 1 (pUNO1-mMD2a)

Genbank : NM_016923.2
ORF size : 483 bp
Subclone : BspLU11I - NheI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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Citations

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