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cGAS (MB21D1)

pUNO1-hCGAS Unit size Cat. code Docs Qty Price
pUNO1 bearing the human cGAS gene
20 µg
puno1-hcgas
+-
$384.00
pUNO1-hCGAS-HA3x Unit size Cat. code Docs Qty Price
pUNO1 bearing the human cGAS-HA gene
20 µg
puno1ha-hcgas
+-
$406.00
pUNO1-hCGAS-AA Unit size Cat. code Docs Qty Price
pUNO1 bearing the human cGAS gene, G212A+S213A inactive mutant
20 µg
puno1-hcgas-aa
+-
$406.00
pUNO1-mCGAS Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse cGAS gene
20 µg
puno1-mcgas
+-
$384.00
pUNO1-mCGAS-HA3x Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse cGAS-HA gene
20 µg
puno1ha-mcgas
+-
$406.00
pUNO1-mCGAS-AA Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse cGAS gene, G212A+S213A inactive mutant
20 µg
puno1-mcgas-aa
+-
$406.00

Expression-ready ORF Clones

Alias : MB21D1, CGAS

 

Cyclic-GMP/AMP synthase.
Mab-21 domain containing 1.

 

cGAS::HA3x :
The cGAS gene has been fused at the 3'end to the influenza hemaglutinine (HA) tag. This short sequence (YPYDVPDYA) encodes a peptide which is the epitope of a very efficient and specific monoclonal antibody. The use of HA-tagged genes provides a simple and convenient method to detect the expression of the genes by Western blot.

Loss of function cGAS mutants :
Two point mutations have been introduced in cGAS: G212A and S213A. These mutations in residues of the NTase active site abolish the activity of full-length cGAS [1,2].

 

1. Civril F, et al. (2013) Nature 498(7454):332-7
2. Marcus A, et al. (2018) Immunity. 49(4):754-763.e4

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Specifications

Human cGAS (pUNO1-hCGAS)

Genbank : NM_138441.3 with one silent mutation in codon Lys500
ORF size : 1569 bp
Subclone : AgeI - AvrII

Mouse cGAS (pUNO1-mCGAS)

Genbank : KC294567.1
ORF size : 1524 bp
Subclone : AgeI - NheI

Human cGAS-HA (pUNO1-hcGAS-HA3x)

ORF size : 1662 bp
Subclone : AgeI - MscI

Mouse cGAS-HA (pUNO1-mcGAS-HA3x)

ORF size : 1617 bp
Subclone : AgeI - NheI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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