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GFP::Sh is a fusion gene that encodes a red-shifted variant of the jellyfish green fluorescent protein (GFP) and the Sh ble gene conferring resistance to Zeocin™. Expression of GFP::Sh can be detected by fluorescent microscopy or flow cytometry. This fusion protein absorbs blue light (major peak at 480 nm) and emits green light (major peak at 505 nm).
The CpG-free GFP::Sh fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in both mammalian and E.coli cells. To facilitate subcloning, the GFP::Sh gene is flanked by two unique restriction sites.
110 CpG in the native geneBack to the top