Poly(I:C) (HMW) Cy3 - TLR3 agonist

Cy3 labeled poly(I:C) HMW

Poly(I:C) HMW Cy3 is a fluorescent labeled synthetic analog of double-stranded RNA (dsRNA). This pathogen associated molecular pattern - short PAMP - is associated with viral infection and a strong ligand for the Toll-like receptor 3 (TLR3).  

Polyinosinic-polycytidylic acid high-molecular weight (Poly(I:C) HMW) was chemically labeled by direct covalent coupling of a Cyanine 3 (Cy3) probe containing a reactive alkylating group onto to a reactive heteroatom within the nucleic acids. This confers fluorescent properties to Poly(I:C) HMW whilst retaining TLR3 recognition properties (see figure). 

Cyanine 3 (Cy3) is a bright, orange-fluorescent dye with excitation suited for the 532 nm laser line. Its stability and brightness makes Cy3 a great tool to use for imaging, flow cytometry, and other fluorescence-based applications (see figures).

In order to facilitate the intracellular delivery, the ligand should be complexed with a cationic lipid transfection agent, such as LyoVec™.

Cy3® is a registered trademark of GE Healthcare.
Cy™ and CyDye™ are registered trademarks of Cytiva.