Rat lambda 1
Rat lambda 1
Light chain expression plasmid - Rat lambda 1
pFUSE2-CLIg-ratL1 is a cloning plasmid that expresses the constant region of the rat lambda 1 light chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the light chain variable region.
This plasmid is designed for antibody generation when co-transfected into mammalian cells with the heavy chain variable region cloned into pFUSE-CHIg.
pFUSE2ss-CLIg-ratL1 additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv) fragments selected from phage display libraries that lack a signal sequence.Back to the top
- pFUSE2-CLIg-ratL1 features the constant region of the mouse Ig lambda 1 light chain
- pFUSE2ss-CLIg-ratL1 features the constant region of the mouse Ig lambda 1 light chain and the IL2 signal sequence
Both plasmids are selectable with blasticidin.Back to the top
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.Back to the top