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pDUO-TLR6/TLR2

pDUO-hTLR6/TLR2 Unit size Cat. code Docs Qty Price
pDUO bearing human TLR6/TLR2 Gene
20 µg
pduo-htlr6tlr2
+-
$464.00
pDUO-mTLR6/TLR2 Unit size Cat. code Docs Qty Price
pDUO bearing mouse TLR6/TLR2 Gene
20 µg
pduo-mtlr6tlr2
+-
$464.00

pDUO-TLR6/TLR2 is an expression vector designed to co-express TLR6 and TLR2 genes known to interact with each other : human TLR6 (2388 bp) and human TLR2 (2352 bp) or murine TLR6 (2418 bp) and murine TLR2 (2352 bp).

The genes cloned into pDUO comprise the coding sequence (without introns) from the ATG to the Stop codon.

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Specifications

pDUO plasmid with human TLR6 and human TLR2 genes.
Name: TLR6 / TLR2
Species:
Human
Backbone: pDUO
Selection: Blasticidin

pDUO plasmid with murine TLR6 and murine TLR2 genes.
Name: TLR6 / TLR2
Species:
Mouse
Backbone: pDUO
Selection: Blasticidin

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Contents

- 20 µg of lyophilized DNA
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)

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Description

TLR6 is expressed in spleen and BPL and, similarly to TLR1, acts as a co-receptor. Studies with dominant negative receptors have shown that TLR6 cooperates with TLR2 to recognize peptidoglycan and the yeast cell wall particle, zymosan [1].
Furthermore, TLR6- and TLR2-deficient mice were reported to be hyporesponsive to mycoplasma macrophage-activating lipopeptide-2 kD (MALP-2), a diacylated lipoprotein, suggesting that TLR2 and TLR6 coordinate the response to this ligand. By contrast, TLR2 is able to recognize bacterial lipoproteins triacylated at the N-terminus cysteine residue [2]. Thus TLR6 appears to discriminate between the N-terminal lipoylated structures of MALP-2 and lipopeptides derived from other bacteria.

1. Ozinsky A. et al. (2000). The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between Toll-like receptors. PNAS 97(25):13766-71.
2. Takeuchi O. et al., 2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int Immunol, 13(7): 933-40.

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Details

pDUO backbone

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