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pDUO-MD1/RP105

pDUO-hMD1/RP105 Unit size Cat. code Docs Qty Price
pDUO bearing human MD1/RP105 Gene
20 µg
pduo-hmd1rp105
+-
$480.00
pDUO-mMD1/RP105 Unit size Cat. code Docs Qty Price
pDUO bearing mouse MD1/RP105 Gene
20 µg
pduo-mmd1rp105
+-
$480.00

pDUO-MD1/RP105 is an expression vector designed to co-express MD1 and RP105 genes known to interact with each other : human MD1 (486 bp) and human RP105 (1983 bp) or murine MD1 (486 bp) and murine RP105 (1983 bp).

The genes cloned into pDUO comprise the coding sequence (without introns) from the ATG to the Stop codon.

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Specifications

pDUO plasmid with human MD1 and human RP105 genes.
Name: MD1 / RP105
Species:
Human
Backbone: pDUO
Selection: Blasticidin

pDUO plasmid with murine MD1 and murine RP105 genes.
Name: MD1 / RP105
Species:
Mouse
Backbone: pDUO
Selection: Blasticidin

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Contents

- 20 µg of lyophilized DNA
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)

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Description

MD1 and RP105 (CD180) are physically associated with each other and are involved in the response to LPS. RP105 is a TLR-related protein, that acts as both an LPS sensor and a regulator of B cell proliferation [1]. Stable expression of MD-1 was shown to induce an increase in cell surface RP105 on a cell line that expresses RP105 alone, suggesting that MD1 is important for efficient cell surface expression of RP105 [2]. When induced by LPS, the RP105/MD1 complex regulates B-cell proliferation, antibody production, and B7.2/CD86 up-regulation [3].

1. Miyake K. et al., 2000. Innate recognition of lipopolysaccharide by Toll-like receptor 4/MD-2 and RP105/MD-1. J Endotoxin Res, 6(5):389-91.
2. Miyake K. et al., 1998. Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol, 161(3):1348-53.
3. Nagai Y. et al., 2002. Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood, 99(5):1699-705.

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Details

pDUO backbone

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